SAE1 cell lysate

Human SAE1 Baculovirus-Insect cells Overexpression Lysate

Gene Names

SAE1; AOS1; SUA1; UBLE1A; HSPC140

Applications

Western Blot

Synonyms

SAE1; Human SAE1 Baculovirus-Insect cells Overexpression Lysate; Human SAE1 Insect Cell Lysate (WB positive control); SUMO1 activating enzyme subunit 1; Human AOS1 Overexpression Lysate; Human HSPC140 Overexpression Lysate; Human SUA1 Overexpression Lysate; Human UBLE1A Overexpression Lysate; SAE1 cell lysate

Ordering
Cat. Num. AAA8115306 For Research Use Only!

Host

Baculovirus-Insect cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Sequence Positions

583aa

Sequence

Met1-Lys346

Applicable Applications for SAE1 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human SAE1 (Q9UBE0) (Met1-Lys346) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Related Product Information for SAE1 cell lysate

SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.

This Human SAE1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SAE1 protein from the overexpression lysate was verified.

References

Gong L, et al. (1999) Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448(1):185-9.Lois LM, et al. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1. EMBO J. 24(3):439-51.Tatham MH, et al. (2001) Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J Biol Chem. 276(38):35368-74.

NCBI and Uniprot Product Information

NCBI GI #

225543285

NCBI GeneID

10055

NCBI Accession #

NP_001139185.1

NCBI GenBank Nucleotide #

NM_001145713.1

UniProt Accession #

Q9UBE0; O95717; Q9P020; B2RDP5; B3KMQ2; F5GXX7; G3XAK6

Molecular Weight

38,450 Da

NCBI Official Full Name

SUMO-activating enzyme subunit 1 isoform b

NCBI Official Synonym Full Names

SUMO1 activating enzyme subunit 1

NCBI Official Symbol

SAE1

NCBI Official Synonym Symbols

AOS1; SUA1; UBLE1A; HSPC140

NCBI Protein Information

SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme

UniProt Protein Name

SUMO-activating enzyme subunit 1

Protein Family

SUMO-activating enzyme

UniProt Gene Name

SAE1

UniProt Synonym Gene Names

AOS1; SUA1; UBLE1A

UniProt Entry Name

SAE1_HUMAN

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Uniprot Description

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytosol; nucleus

Molecular Function: protein C-terminus binding; ATP-dependent protein binding; SUMO activating enzyme activity; protein binding; protein heterodimerization activity; enzyme activator activity; ubiquitin activating enzyme activity

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; regulation of mitotic cell cycle; protein ubiquitination; SMT3-dependent protein catabolic process; post-translational protein modification

Research Articles on SAE1

1. Data indicate the role of anti-SUMO activating enzyme SAE1 and SAE2 antibody as marker of dermatomyositis.

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Product Notes
The SAE1 sae1 (Catalog #AAA8115306) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 583aa. AAA Biotech's SAE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SAE1 sae1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys34 6. It is sometimes possible for the material contained within the vial of "SAE1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.