S100A6 cell lysate

Human S100A6 Baculovirus-Insect cells Overexpression Lysate

Gene Names

S100A6; 2A9; PRA; 5B10; CABP; CACY

Applications

Western Blot

Synonyms

S100A6; Human S100A6 Baculovirus-Insect cells Overexpression Lysate; Human S100A6/CACY Insect Cell Lysate (WB positive control); S100 calcium binding protein A6; Human 2A9 Overexpression Lysate; Human 5B10 Overexpression Lysate; Human CABP Overexpression Lysate; Human CACY Overexpression Lysate; Human PRA Overexpression Lysate; S100A6 cell lysate

Ordering
Cat. Num. AAA8114242 For Research Use Only!

Host

Baculovirus-Insect cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Sequence Positions

101aa

Sequence

Met1-Gly90

Applicable Applications for S100A6 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human S100A6 (NP_055439.1) (Met 1-Gly 90) was expressed, with a C-terminal polyhistidine tag.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Related Product Information for S100A6 cell lysate

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A6 (S100 calcium-binding protein A6) is a member of the S100 family of proteins, and functions in prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 have been implicated in melanoma.

This Human S100A6 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of S100A6 protein from the overexpression lysate was verified.

Product Categories/Family for S100A6 cell lysate

Calcium-binding Proteins and Related Molecules

References

Schafer, B.W. et al., 1996, Trends Biochem. Sci. 21 (4): 134-140. Donato, R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-551. Nowotny, M. et al., 2003, J. Biol. Chem. 278 (29): 26923-26928. Nonaka D, et al., 2008, J. Cutan. Pathol. 35 (11): 1014-1019. Marenholz, I. et al., 2004, Biochem. Biophys. Res. Commun. 322 (4): 1111-22.

NCBI and Uniprot Product Information

NCBI GI #

7657532

NCBI GeneID

6277

NCBI Accession #

NP_055439.1

NCBI GenBank Nucleotide #

NM_014624.3

UniProt Accession #

P06703; Q5RHS4; D3DV39

Molecular Weight

10,180 Da

NCBI Official Full Name

protein S100-A6

NCBI Official Synonym Full Names

S100 calcium binding protein A6

NCBI Official Symbol

S100A6

NCBI Official Synonym Symbols

2A9; PRA; 5B10; CABP; CACY

NCBI Protein Information

protein S100-A6; MLN 4; S100 calcium-binding protein A6 (calcyclin); growth factor-inducible protein 2A9; prolactin receptor-associated protein

UniProt Protein Name

Protein S100-A6

Protein Family

Protein

UniProt Gene Name

S100A6

UniProt Synonym Gene Names

CACY; PRA

UniProt Entry Name

S10A6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A6: May function as calcium sensor and contribute to cellular calcium signaling (Potential). May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: ruffle; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; ion transmembrane transporter activity; calcium ion binding; tropomyosin binding; calcium-dependent protein binding

Biological Process: positive regulation of fibroblast proliferation; axonogenesis; signal transduction

Research Articles on S100A6

1. In Wharton's jelly of preeclamptic tissue S100 calcium binding protein A6(S100A6) is up-regulated and binds to different targets than in control suggesting involvement in development of preeclampsia

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Product Notes
The S100A6 s100a6 (Catalog #AAA8114242) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 101aa. AAA Biotech's S100A6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the S100A6 s100a6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly90. It is sometimes possible for the material contained within the vial of "S100A6, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.