ENTPD2 cell lysate
Human ENTPD2 Baculovirus-Insect cells Overexpression Lysate
Gene Names
ENTPD2; CD39L1; NTPDase-2
Applications
Western Blot
Synonyms
ENTPD2; Human ENTPD2 Baculovirus-Insect cells Overexpression Lysate; Human NTPDase 2/ENTPD2 (aa 29-460) Insect Cell Lysate (WB positive control); ectonucleoside triphosphate diphosphohydrolase 2; Human CD39L1 Overexpression Lysate; Human NTPDase-2 Overexpression Lysate; Human RP11-229P13.11-001 Overexpression Lysate; ENTPD2 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
448aa
Sequence
Thr29-Asp460
Applicable Applications for ENTPD2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mature form of human ENTPD2 (Q9Y5L3) (Thr29-Asp460) was expressed, with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ENTPD2 cell lysate
NTPDase 2, also known as ENTPD2, belongs to the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). Members of E-NTPDase family are nucleotidases able to hydrolyze 5'-nucleoside tri-and/or diphosphates; the main role of these enzymes is the termination of purinergic signaling. NTPDases are ubiquitous and were previously shown in other parasites including the trypanosomatides of genus Leishmania and in T. brucei. NTPase activity would act as a timer and is crucial to T. gondii infection. In L. pneumophila it was demonstrated that an E-NTPDase, similar to CD39, is essential for intracellular bacterial multiplication. NTPDase 2 is an integral membrane protein. In the nervous system, it could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Alternative splicing of NTPDase 2 gene results in multiple transcript variants.
This Human ENTPD2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of ENTPD2 protein from the overexpression lysate was verified.
This Human ENTPD2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of ENTPD2 protein from the overexpression lysate was verified.
References
Harden TK, et al. (2005) Regulation of P2Y1 receptor-mediated signaling by the ectonucleoside triphosphate diphosphohydrolase isozymes NTPDase1 and NTPDase2. Mol Pharmacol. 67(1): 114-22.Dranoff JA, et al. (2005) Ectonucleotidase NTPDase2 is selectively down-regulated in biliary cirrhosis. J Investig Med. 52(7):475-82.Jhandier MN, et al.. (2005) Portal fibroblasts regulate the proliferation of bile duct epithelia via expression of NTPDase2. J Biol Chem. 280(24):22986-92.Mukasa T, et al. (2005) Either the carboxyl-or the amino-terminal region of the human ecto-ATPase (E-NTPDase 2) confers detergent and temperature sensitivity to the chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8). Biochemistry. 44(33):11160-70.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
495
NCBI Official Full Name
Ectonucleoside triphosphate diphosphohydrolase 2
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 2
NCBI Official Symbol
ENTPD2
NCBI Official Synonym Symbols
CD39L1; NTPDase-2
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 2; NTPDase 2; ecto-ATPase 2; ecto-ATPDase 2; CD39 antigen-like 1; ecto-ATP diphosphohydrolase 2
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 2
Protein Family
UniProt Gene Name
ENTPD2
UniProt Synonym Gene Names
CD39L1; NTPDase 2; Ecto-ATPDase 2; Ecto-ATPase 2
UniProt Entry Name
ENTP2_HUMAN
NCBI Description
The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
ENTPD2: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP. Belongs to the GDA1/CD39 NTPase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.6.1.-; Nucleotide Metabolism - purine; Extracellular matrix; Membrane protein, integral; Membrane protein, multi-pass; Hydrolase
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane; basal lamina
Molecular Function: nucleoside-diphosphatase activity; nucleoside-triphosphatase activity; ATP binding
Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; purine ribonucleoside diphosphate catabolic process
Research Articles on ENTPD2
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Product Notes
The ENTPD2 entpd2 (Catalog #AAA8114754) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 448aa. AAA Biotech's ENTPD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ENTPD2 entpd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Thr29-Asp4 60. It is sometimes possible for the material contained within the vial of "ENTPD2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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