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CDK7 & CCNH & MNAT1 Cell Lysate | CDK7 cell lysate

Human CDK7 & CCNH & MNAT1 Baculovirus-Insect cells Overexpression Lysate

Gene Names
CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
Applications
Western Blot
Synonyms
CDK7 & CCNH & MNAT1; Human CDK7 & CCNH & MNAT1 Baculovirus-Insect cells Overexpression Lysate; Human CDK7 & CCNH & MNAT1 Heterotrimer Insect Cell Lysate (WB positive control); cyclin-dependent kinase 7; Human CAK1 Overexpression Lysate; Human CDKN7 Overexpression Lysate; Human HCAK Overexpression Lysate; Human MO15 Overexpression Lysate; Human p39MO15 Overexpression Lysate; Human STK1 Overexpression Lysate; CDK7 cell lysate
Ordering
For Research Use Only!
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
1032 (364 + 341 + 327)aa
Sequence
Ala2-Phe346, Tyr2-Leu323, Asp2-Ser309
Applicable Applications for CDK7 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CDK7 cell lysate
This Human CDK7 & CCNH & MNAT1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CDK7 & CCNH & MNAT1 protein from the overexpression lysate was verified.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,038 Da
NCBI Official Full Name
cyclin-dependent kinase 7
NCBI Official Synonym Full Names
cyclin-dependent kinase 7
NCBI Official Symbol
CDK7
NCBI Official Synonym Symbols
CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
NCBI Protein Information
cyclin-dependent kinase 7; CAK; p39 Mo15; protein kinase; 39 KDa protein kinase; kinase subunit of CAK; CDK-activating kinase 1; serine/threonine kinase stk1; cell division protein kinase 7; serine/threonine protein kinase 1; serine/threonine-protein kina
UniProt Protein Name
Cyclin-dependent kinase 7
Protein Family
UniProt Gene Name
CDK7
UniProt Synonym Gene Names
CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15
UniProt Entry Name
CDK7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK7: a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle. Phosphorylates and activates RNA polymerase II, allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; Protein kinase, CMGC; EC 2.7.11.23; CMGC group; CDK family; CDK7 subfamily; CDK/CDK7 subfamily

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: nucleoplasm; mitochondrion; holo TFIIH complex; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; androgen receptor binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; G2/M transition of mitotic cell cycle; cell cycle arrest; transcription initiation from RNA polymerase II promoter; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; cell proliferation; nucleotide-excision repair; cell division; RNA elongation from RNA polymerase II promoter; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; transcription initiation from RNA polymerase I promoter; G1/S transition of mitotic cell cycle

Research Articles on CDK7

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Product Notes

The CDK7 cdk7 (Catalog #AAA8118073) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1032 (364 + 341 + 327)aa. AAA Biotech's CDK7 & CCNH & MNAT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CDK7 cdk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala2-Phe34 6, Tyr2-Leu32 3, Asp2-Ser30 9. It is sometimes possible for the material contained within the vial of "CDK7 & CCNH & MNAT1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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