c-Abl/ABL1 Cell Lysate | ABL1 cell lysate
Human c-Abl/ABL1 Baculovirus-Insect cells Overexpression Lysate
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Samples are stable for up to twelve months from date of receipt.
This Human c-Abl/ABL1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of c-Abl/ABL1 protein from the overexpression lysate was verified.
Huang Y, et al. (2008) The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse. Blood. 112(1): 111-9.
Sirvent A, et al. (2008) Cytoplasmic signalling by the c-Abl tyrosine kinase in normal and cancer cells. Biol Cell. 100(11): 617-31.
Shaul Y, et al. (2005) Role of c-Abl in the DNA damage stress response. Cell Res. 15(1): 33-5.
NCBI and Uniprot Product Information
NCBI Description
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]
Uniprot Description
Abl: an ubiquitously expressed and highly conserved proto-oncogenic tyrosine kinase. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. Negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response. The Philadephia chromosome translocation t(9;22)(q34;q11) creates a Bcr-Abl fusion protein, responsible for 90% of chronic myelogenous leukemia (CML) and ~25% of acute lymphoblastic leukemia (ALL). Inhibitors: Gleevec (imatinib, Glivec), Dasatinib. Two alternatively-spliced isoforms have been described.
Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; Oncoprotein; EC 2.7.10.2; TK group; Abl family
Chromosomal Location of Human Ortholog: 9q34.1
Cellular Component: nucleoplasm; nuclear membrane; extrinsic to internal side of plasma membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; leading edge; nucleolus; nucleus; cytosol; actin cytoskeleton
Molecular Function: protein C-terminus binding; actin monomer binding; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; protein kinase activity; actin filament binding; protein binding; DNA binding; syntaxin binding; protein-tyrosine kinase activity; manganese ion binding; mitogen-activated protein kinase binding; nicotinate-nucleotide adenylyltransferase activity; SH3 domain binding; ATP binding; receptor binding
Biological Process: DNA damage induced protein phosphorylation; positive regulation of apoptosis; cerebellum morphogenesis; negative regulation of BMP signaling pathway; elevation of cytosolic calcium ion concentration; transitional one stage B cell differentiation; B cell receptor signaling pathway; regulation of endocytosis; negative regulation of mitotic cell cycle; cell cycle arrest; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; spleen development; mitosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of peptidyl-tyrosine phosphorylation; muscle cell differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of osteoblast proliferation; negative regulation of ubiquitin-protein ligase activity; autophagy; response to oxidative stress; actin cytoskeleton organization and biogenesis; axon guidance; peptidyl-tyrosine phosphorylation; alpha-beta T cell differentiation; platelet-derived growth factor receptor signaling pathway; mitochondrial depolarization; positive regulation of mitotic cell cycle; Bergmann glial cell differentiation; regulation of transcription, DNA-dependent; collateral sprouting; cell differentiation; B cell proliferation during immune response; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; cell migration; mismatch repair; thymus development; DNA damage response, signal transduction; B-1 B cell homeostasis; activated T cell proliferation; protein modification process; regulation of cell proliferation; microspike biogenesis; innate immune response; positive regulation of muscle cell differentiation; regulation of autophagy; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of cell-cell adhesion; blood coagulation; response to DNA damage stimulus
Disease: Leukemia, Chronic Myeloid
Research Articles on ABL1
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Product Notes
The ABL1 abl1 (Catalog #AAA8114469) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 645aa. AAA Biotech's c-Abl/ABL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ABL1 abl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Pro137-Ser 554. It is sometimes possible for the material contained within the vial of "c-Abl/ABL1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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