15 Lipoxygenase 2 Cell Lysate | ALOX15B cell lysate
Human 15 Lipoxygenase 2 Baculovirus-Insect cells Overexpression Lysate
Applications
Western Blot
Synonyms
15 Lipoxygenase 2; Human 15 Lipoxygenase 2 Baculovirus-Insect cells Overexpression Lysate; Human ALOX15B/15 Lipoxygenase 2 Insect Cell Lysate (WB positive control); arachidonate 15-lipoxygenase; type B; Human 15-LOX-2 Overexpression Lysate; Human ALOX15B Overexpression Lysate; ALOX15B cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
913aa
Sequence
Met1-Ile676
Applicable Applications for ALOX15B cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human ALOX15B (AAH35217.1) (Met1-Ile676) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ALOX15B cell lysate
ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 1 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
This Human 15 Lipoxygenase 2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of 15 Lipoxygenase 2 protein from the overexpression lysate was verified.
This Human 15 Lipoxygenase 2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of 15 Lipoxygenase 2 protein from the overexpression lysate was verified.
References
Kilty I. et al., 2000, Eur J Biochem. 266 (1): 83-93. Sigal E. et al., 1990, J Biol Chem. 265 (9): 5113-20. Brash AR. et al., 1997, Proc Natl Acad Sci. 94 (12): 6148-52.
NCBI and Uniprot Product Information
Molecular Weight
The secreted recombinant human ALOX15B consists of 913 amino acids and predicts a molecular mass of 103.7kDa.
The apparent molecular mass of the protein is approximately 93kDa in SDS-PAGE under reducing conditions due to glycosylation.
The apparent molecular mass of the protein is approximately 93kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein Family
Similar Products
Product Notes
The ALOX15B (Catalog #AAA8115400) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 913aa. AAA Biotech's 15 Lipoxygenase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ALOX15B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ile67 6. It is sometimes possible for the material contained within the vial of "15 Lipoxygenase 2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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