CCNE1 / Cyclin-E1 Active Protein | CCNE1 active protein

Recombinant Human CCNE1/Cyclin-E1 Protein (His Tag)(Active)

Cat. Num. AAA2546021

• Gene Names: CCNE1; CCNE; pCCNE1
• Purity: > 85 % as determined by reducing SDS-PAGE.
• Synonyms: CCNE1 / Cyclin-E1; Recombinant Human CCNE1/Cyclin-E1 Protein (His Tag)(Active); CCNE; CCNE1; CCNE1 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.5, 10% glycerol, pH 7.5, 5%Trehalose, 5% Mannitol, 0.01% Tween 80.
• Sequence: Met 1-Ala 410

• Application Notes: The recombinant human CCNE1 consists of 429 amino acids and predicts a molecular mass of 49.3 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Species: Human
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Tag: N-His
• Bio_activity: Measured by its binding ability in a functional ELISA. Immobilized human CCNE1 at 10 ug/ml (100 ul/well) can bind biotinylated human CDK4. The EC50 of biotinylated human CDK4 is 0.10-0.32 ug/ml.
• Reconstitution: It is recommended that sterile water (800uL) be added to the vial to prepare a stock solution of 0.125mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-PAGE

Related Product Information for CCNE1 active protein

Background: Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Description: A DNA sequence encoding the full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 17318559
• NCBI GeneID: 898
• NCBI Accession #: NP_001229.1
• NCBI GenBank Nucleotide #: NM_001238.3
• UniProt Accession #: P24864; Q14091; Q8NFG1; Q92501; Q9UD21; A8K684
• Molecular Weight: Mol_Mass: 49.3 kDa; AP_Mol_Mass: 47 kDa
• NCBI Official Full Name: G1/S-specific cyclin-E1 isoform 1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: CCNE1
• NCBI Official Synonym Symbols: CCNE; pCCNE1
• NCBI Protein Information: G1/S-specific cyclin-E1
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: CCNE1
• UniProt Synonym Gene Names: CCNE

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Activator; Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; protein amino acid phosphorylation