CD48 active protein

CD48, 27-220aa, Human, His-tag, Baculovirus

Cat. Num. AAA206692

• Gene Names: CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CD48; 27-220aa; Human; His-tag; Baculovirus; CD48 antigen isoform 1; BCM1; BLAST; BLAST1; hCD48; mCD48; MEM-102; SLAMF2; CD48 active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 1.0mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPQGHLVHM TVVSGSNVTL NISESLPENY KQLTWFYTFD QKIVEWDSRK SKYFESKFKG RVRLDPQSGA LYISKVQKED NSTYIMRVLK KTGNEQEWKI KLQVLDPVPK PVIKIEKIED MDDNCYLKLS CVIPGESVNY TWYGDKRPFP KELQNSVLET TLMPHNYSRC YTCQVSNSVS SKNGTVCLSP PCTLARSHHH HHH
• Sequence Length: 243

• Applicable Applications for CD48 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CD48 active protein

CD48, also known as CD48 antigen isoform 1, is a GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules. This protein is competent to transduce signals and can also trigger signaling through CD2 or 2B4. CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells. High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation. It expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation. Recombinant human CD48, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD48 active protein

Immunology

References

Kis-Toth K., et al, (2014) J. Immunol. 192:4436-4442.; Fisher RC., et al, (1991) Mol. Cell. Biol. 11:1614-1623.

NCBI and Uniprot Product Information

• NCBI GI #: 21361571
• NCBI GeneID: 962
• NCBI Accession #: NP_001769
• NCBI GenBank Nucleotide #: NM_001778.3
• UniProt Accession #: P09326; Q5U055; Q8MGR0
• Molecular Weight: 23.4kDa (203aa) 28-40kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: CD48 antigen isoform 1
• NCBI Official Synonym Full Names: CD48 molecule
• NCBI Official Symbol: CD48
• NCBI Official Synonym Symbols: BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• NCBI Protein Information: CD48 antigen
• UniProt Protein Name: CD48 antigen
• Protein Family: CD48 antigen
• UniProt Gene Name: CD48
• UniProt Synonym Gene Names: BCM1; BLAST1; SLAMF2

NCBI Description

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.