B3GAT1 recombinant protein

Recombinant Human B3GAT1 (N-6His)

Cat. Num. AAA2569238

• Gene Names: B3GAT1; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: B3GAT1; Recombinant Human B3GAT1 (N-6His); Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; beta-1; 3-glucuronyltransferase 1 (glucuronosyltransferase P); CD57; GlcAT-P; HNK1; NK1; NK-1; B3GAT1 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of 20mM Tris-HCl, 150mM NaCl, pH8.0.
• Sequence: His25-Ile334

• Species: Human
• Tag: N-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 is produced by our Mammalian expression system and the target gene encoding His25-Ile334 is expressed with a 6His tag at the N-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for B3GAT1 recombinant protein

B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc. The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system. Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method.

Product Categories/Family for B3GAT1 recombinant protein

immunology

NCBI and Uniprot Product Information

• NCBI GI #: 77695914
• NCBI GeneID: 27087
• NCBI Accession #: NP_061114.2
• NCBI GenBank Nucleotide #: NP_061114.2
• UniProt Accession #: Q9P2W7
• NCBI Official Full Name: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: beta-1,3-glucuronyltransferase 1
• NCBI Official Symbol: B3GAT1
• NCBI Official Synonym Symbols: NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• NCBI Protein Information: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• UniProt Protein Name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• Protein Family: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
• UniProt Gene Name: B3GAT1
• UniProt Synonym Gene Names: GlcUAT-P

NCBI Description

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.