AXL Receptor Tyrosine Kinase Recombinant Protein | AXL recombinant protein

Recombinant AXL Receptor Tyrosine Kinase (AXL)

Cat. Num. AAA2029010

• Gene Names: AXL; ARK; UFO; JTK11; Tyro7
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: AXL Receptor Tyrosine Kinase; Recombinant AXL Receptor Tyrosine Kinase (AXL); AXL recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; KDTQTEADSP FVGNPGNITG ARGLTGTLRCELQVQGEPPE VMWLRDGQIL ELADNTQTQV PLGEDWQDEW KVVSQLRISALQLSDAGEYQ CMVHLEGRTF VSQPGFVGLE GLPYFLEEPE DKAVPANTPFNLSCQAQGPP EPVTLLWLQD AVPLAPVAGY SFQHSLQAPG LNKTSSFSCEAH
• Sequence Length: 894

• Applicable Applications for AXL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 23.5kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Lys21~His202
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21619004
• NCBI GeneID: 558
• Molecular Weight: 97,377 Da
• NCBI Official Full Name: AXL receptor tyrosine kinase
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: AXL
• NCBI Official Synonym Symbols: ARK; UFO; JTK11; Tyro7
• NCBI Protein Information: tyrosine-protein kinase receptor UFO
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: AXL
• UniProt Synonym Gene Names: UFO
• UniProt Entry Name: UFO_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Oncoprotein; Membrane protein, integral; Protein kinase, TK; TK group; Axl family

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; phosphatidylserine binding; protein heterodimerization activity; protein-tyrosine kinase activity; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding

Biological Process: peptidyl-tyrosine phosphorylation; cell maturation; neuron migration; signal transduction; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; negative regulation of neuron apoptosis; inflammatory response; secretion by cell; forebrain cell migration; platelet activation; negative regulation of lymphocyte activation; organ regeneration; vagina development; negative regulation of tumor necrosis factor production; phagocytosis; positive regulation of protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; blood vessel remodeling; spermatogenesis; positive regulation of cytokine and chemokine mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway; apoptotic cell clearance