Monkey Axis Inhibition Protein 2 ELISA Kit | AXIN2 elisa kit

Monkey Axis Inhibition Protein 2 ELISA Kit

Cat. Num. AAA751935

• Gene Names: AXIN2; AXIL; ODCRCS
• Reactivity: Monkey
• Synonyms: Axis Inhibition Protein 2; Monkey Axis Inhibition Protein 2 ELISA Kit; AXIN2 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AXIN2 elisa kit

Principle of the assay: AXIN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AXIN2 antibody and an AXIN2-HRP conjugate. The assay sample and buffer are incubated together with AXIN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AXIN2 concentration since AXIN2 from samples and AXIN2-HRP conjugate compete for the anti-AXIN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AXIN2 from the sample, fewer sites are left to bind AXIN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AXIN2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AXIN2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 195927059
• NCBI GeneID: 8313
• NCBI Accession #: NP_004646.3
• NCBI GenBank Nucleotide #: NM_004655.3
• Molecular Weight: 93,558 Da
• NCBI Official Full Name: axin-2
• NCBI Official Synonym Full Names: axin 2
• NCBI Official Symbol: AXIN2
• NCBI Official Synonym Symbols: AXIL; ODCRCS
• NCBI Protein Information: axin-2; conductin; axin-like protein; axis inhibition protein 2
• UniProt Protein Name: Axin-2
• Protein Family: Axin
• UniProt Gene Name: AXIN2
• UniProt Synonym Gene Names: Axil
• UniProt Entry Name: AXIN2_HUMAN

NCBI Description

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

Uniprot Description

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: Golgi apparatus; centrosome; cytoplasmic membrane-bound vesicle; postsynaptic density; cell cortex; cytosol; beta-catenin destruction complex; nucleoplasm; microtubule cytoskeleton; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; cell proliferation; negative regulation of cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; dorsal/ventral axis specification; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome