SDS-Page
Preprovasopressin (VP) Recombinant Protein | VP recombinant protein
Recombinant Preprovasopressin (VP)
Gene Names
Avp; DI; VP; Vas
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Preprovasopressin (VP); Recombinant Preprovasopressin (VP); VP recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-CYFQNCPRGG KRATSDMELR QCLPCGPGGK GRCFGPSICC ADELGCFLGT AEALRCQEEN YLPSPCQSGQ KPCGSGGRCA AAGICCSDES CVAEPECREG FFRLTRAREQ SNATQLDGPA RELLLRLVQL AGTQESVDSA KPRVY
MGHHHHHHSGSEF-CYFQNCPRGG KRATSDMELR QCLPCGPGGK GRCFGPSICC ADELGCFLGT AEALRCQEEN YLPSPCQSGQ KPCGSGGRCA AAGICCSDES CVAEPECREG FFRLTRAREQ SNATQLDGPA RELLLRLVQL AGTQESVDSA KPRVY
Sequence Length
168
Applicable Applications for VP recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Cys24~Tyr268 (Accession # P01186) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17.0kDa
NCBI Official Full Name
vasopressin-neurophysin 2-copeptin preproprotein
NCBI Official Synonym Full Names
arginine vasopressin
NCBI Official Symbol
Avp
NCBI Official Synonym Symbols
DI; VP; Vas
NCBI Protein Information
vasopressin-neurophysin 2-copeptin; AVP-NPII; prepropressophysin; preprovasopressin-neurophysin; vasopressin-neurophysin prepropeptide; arginine vasopressin (Diabetes insipidus), same as Di (conflicting physical mapping)
UniProt Protein Name
Vasopressin-neurophysin 2-copeptin
UniProt Gene Name
Avp
UniProt Entry Name
NEU2_RAT
NCBI Description
neuropeptide hormone; involved in vasoconstriction to regulate blood pressure and smooth muscle contraction during parturition and lactation [RGD, Feb 2006]
Uniprot Description
Function: Neurophysin 2 specifically binds vasopressin.Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
Subcellular location: Secreted.
Sequence similarities: Belongs to the vasopressin/oxytocin family.
Sequence caution: The sequence AAA42342.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on VP
1. Suggest that the P2X7R is expressed in neurohypophysial terminals and has a role in AVP secretion.
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Product Notes
The VP avp (Catalog #AAA2010729) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Preprovasopressin (VP) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the VP avp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-CYFQNC PRGG KRATSDMELR QCLPCGPGGK GRCFGPSICC ADELGCFLGT AEALRCQEEN YLPSPCQSGQ KPCGSGGRCA AAGICCSDES CVAEPECREG FFRLTRAREQ SNATQLDGPA RELLLRLVQL AGTQESVDSA KPRVY. It is sometimes possible for the material contained within the vial of "Preprovasopressin (VP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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