ATP Synthase Mitochondrial Fo Complex Subunit D Recombinant Protein | ATP5H recombinant protein

Recombinant Human ATP Synthase Mitochondrial Fo Complex Subunit D

Cat. Num. AAA146130

• Gene Names: ATP5H; ATPQ
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: ATP Synthase Mitochondrial Fo Complex Subunit D; Recombinant Human ATP Synthase Mitochondrial Fo Complex Subunit D; ATP5H Human; ATP Synthase Mitochondrial Fo Complex Subunit D Human Recombinant; ATP Synthase H+ Transporting; Mitochondrial Fo Complex Subunit D; ATP Synthase D Chain Mitochondrial; ATP Synthase H+ Transporting Mitochondrial F1F0 Subunit D; ATPase Subunit D; My032 Protein; ATPQ; ATP5H recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: The ATP5H solution contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGRKLA LKTIDWVAFA EIIPQNQKAI ASSLKSWNET LTSRLAALPE NPPAIDWAYY KANVAKAGLV DDFEKKFNAL KVPVPEDKYT AQVDAEEKED VKSCAEWVSL SKARIVEYEK EMEKMKNLIP FDQMTIEDLN EAFPETKLDK KKYPYWPHQP IENL
• Sequence Length: 137

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for ATP5H recombinant protein

Description: ATP5H Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids (1-161) and having a molecular mass of 20.9kDa.ATP5H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: ATP5H is a member of the ATPase d subunit family and encodes the d subunit of the F0 complex. Electron transport complexes of the respiratory chain create a proton gradient across the membrane which induces ATP5H to transform ADP to ATP. Two alternatively spliced isoforms of ATP5H, encoded by a gene that maps to human chromosome 17q25.1, are known. ATP5H is restricted to mitochondrial inner membrane.

Product Categories/Family for ATP5H recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 51479152
• NCBI GeneID: 10476
• NCBI Accession #: NP_001003785.1
• NCBI GenBank Nucleotide #: NM_001003785.1
• UniProt Accession #: O75947; Q9H3J4; B2R5L6
• Molecular Weight: 15,773 Da
• NCBI Official Full Name: ATP synthase subunit d, mitochondrial isoform b
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d
• NCBI Official Symbol: ATP5H
• NCBI Official Synonym Symbols: ATPQ
• NCBI Protein Information: ATP synthase subunit d, mitochondrial; ATP synthase D chain, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATPase subunit d; My032 protein
• UniProt Protein Name: ATP synthase subunit d, mitochondrial
• UniProt Gene Name: ATP5H
• UniProt Synonym Gene Names: ATPase subunit d
• UniProt Entry Name: ATP5H_HUMAN

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]

Uniprot Description

ATP5H: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase d subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; cytoplasm; mitochondrial proton-transporting ATP synthase complex

Molecular Function: ATPase activity; hydrogen ion transmembrane transporter activity; transmembrane transporter activity

Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport