ATPase, Na+/K+ Transporting Beta 4 Polypeptide Recombinant Protein | ATP1b4 recombinant protein

Recombinant ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4)

Cat. Num. AAA2033643

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: ATPase; Na+/K+ Transporting Beta 4 Polypeptide; Recombinant ATPase; Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4); X; K-ATPase subunit beta-m; X/potassium-transporting ATPase subunit beta-m; ATP1b4 recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MLFLAISPY MPTFTEQVKPPGVMIRPFAH SLNFNFNVSE PETWQRYVIS LNGFLQGYND SLQEEMNIDCPPGQYFIQDG DEDEDKKACQ FKRSFLKNCS GLEDPTFGYS TGQPCILLKMNRIVGFRPEF GDPVKVSCKV QKGDENDIRS INYYPESASF DLRYYPYYGKLTHVNYTSPL VAMHFTDVVK NQEVPVQCQL KGKGIVNDVI NDRFVGRIIFTLNIET
• Sequence Length: 353

• Applicable Applications for ATP1b4 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 29.5kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met132~Thr356
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119632291
• NCBI GeneID: 23439
• Molecular Weight: 41,109 Da
• NCBI Official Full Name: ATPase, (Na+)/K+ transporting, beta 4 polypeptide, isoform CRA_b
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 4 polypeptide
• NCBI Official Symbol: ATP1B4
• NCBI Protein Information: protein ATP1B4
• UniProt Protein Name: Protein ATP1B4
• Protein Family: Protein
• UniProt Gene Name: ATP1B4
• UniProt Entry Name: AT1B4_HUMAN

NCBI Description

This gene has been found in all vertebrate genomes sequenced to date. However, this gene has undergone a change in function in placental mammals compared to other species. Specifically, in fish, avian, and amphibian species, this gene encodes plasma membrane-bound beta-subunits of Na,K-ATPase. In placental mammals, the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

ATP1B4: May act as a transcriptional coregulator during muscle development through its interaction with SNW1. Has lost its ancestral function as a Na,K-ATPase beta-subunit. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: chromatin; integral to plasma membrane; nuclear envelope; nuclear inner membrane; sodium:potassium-exchanging ATPase complex

Molecular Function: monovalent inorganic cation transmembrane transporter activity

Biological Process: gene expression; monovalent inorganic cation transport; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; transport