ATP1B2 recombinant protein

ATP1B2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121969

• Gene Names: ATP1B2; AMOG
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ATP1B2; ATP1B2 Protein; Human; Recombinant (His Tag); Human ATP1B2/ATPase Na+/ K+ beta 2 Protein (His Tag); AMOG Protein; ATPase; Na+/K+ transporting; beta 2 polypeptide; ATP1B2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp68-Thr290

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ATP1B2 (NP_001669.3)(Asp68-Thr290) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 49574491
• NCBI GeneID: 482
• NCBI Accession #: NP_001669.3
• NCBI GenBank Nucleotide #: NM_001678.4
• UniProt Accession #: P14415; O60444; A0AV17; A8K278; D3DTQ2
• Molecular Weight: 33,367 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-2 isoform 1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 2 polypeptide
• NCBI Official Symbol: ATP1B2
• NCBI Official Synonym Symbols: AMOG
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-2; Na, K-ATPase beta-2 polypeptide; adhesion molecule in glia; adhesion molecule on glia; sodium pump subunit beta-2; sodium-potassium ATPase subunit beta 2 (non-catalytic); sodium/potassium-dependent ATPa
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-2
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1B2
• UniProt Synonym Gene Names: AMOG
• UniProt Entry Name: AT1B2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

ATP1B2: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Belongs to the X(+)/potassium ATPases subunit beta family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; sodium:potassium-exchanging ATPase complex

Molecular Function: ATPase activator activity; protein binding; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular sodium ion homeostasis; protein stabilization; potassium ion import; transport; positive regulation of ATPase activity; blood coagulation; cell adhesion; leukocyte migration; cellular potassium ion homeostasis; transmembrane transport