ATG4B recombinant protein

ATG4B, 1-393aa, Human, His tag, E Coli

Cat. Num. AAA204719

• Gene Names: ATG4B; APG4B; AUTL1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ATG4B; 1-393aa; Human; His tag; E Coli; Cysteine protease ATG4B; AuTL1; Autophagin-1; ATG4B recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVEQQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSLLEHHHHH H
• Sequence Length: 393

• Applicable Applications for ATG4B recombinant protein: SDS-PAGE
• Tag Information: His-tag
• Domain: 1-393aa
• Preparation and Storage: Can be stored at +2°C to +8°C short term (1 weeks).; For long term storage, aliquot and store at -20°C or -80°C.; Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for ATG4B recombinant protein

ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for ATG4B recombinant protein

Ubiquitination

References

Sugawara K., et al. (2005) J. Biol. Chem. 280:40058-40065; Kumanomidou T., et al. (2006) J. Mol. Biol. 355:612-618;

NCBI and Uniprot Product Information

• NCBI GI #: 12653857
• NCBI GeneID: 23192
• Molecular Weight: 45.3 kDa (401aa) confirmed by MALDI-TOF
• NCBI Official Full Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• NCBI Official Synonym Full Names: autophagy related 4B cysteine peptidase
• NCBI Official Symbol: ATG4B
• NCBI Official Synonym Symbols: APG4B; AUTL1
• NCBI Protein Information: cysteine protease ATG4B
• UniProt Protein Name: Cysteine protease ATG4B
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4B
• UniProt Synonym Gene Names: APG4B; AUTL1; KIAA0943; hAPG4B

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.