Mouse Aspartate Aminotransferase ELISA Kit | AST elisa kit

Mouse Aspartate Aminotransferase ELISA Kit

Cat. Num. AAA760475

• Gene Names: ASP5; AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• Reactivity: Mouse
• Synonyms: Aspartate Aminotransferase; Mouse Aspartate Aminotransferase ELISA Kit; AST; AST elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of AST. No significant cross-reactivity or interference between AST and analogues was observed.
• Sequence Length: 448

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AST were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AST were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AST elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AST antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AST antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AST amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AST can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 334187077
• NCBI GeneID: 829330
• NCBI Accession #: NP_001190885.1
• NCBI GenBank Nucleotide #: NM_001203956.1
• Molecular Weight: 49,831 Da
• NCBI Official Full Name: aspartate aminotransferase
• NCBI Official Symbol: ASP5
• NCBI Official Synonym Symbols: AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• NCBI Protein Information: aspartate aminotransferase
• UniProt Protein Name: Aspartate aminotransferase, chloroplastic
• Protein Family: Allatostatin
• UniProt Gene Name: ASP5
• UniProt Entry Name: AAT5_ARATH

NCBI Description

encodes a plastid-localized aspartate aminotransferase

Uniprot Description

Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.