Testing Data
Rapid GST Inclusion Body Solubilization and Renaturation Kit Assay Kit
Rapid GST Inclusion Body Solubilization and Renaturation Kit
Synonyms
Rapid GST Inclusion Body Solubilization and Renaturation Kit; Rapid GST Inclusion Body Solubilization and Renaturation Kit assay kit
Preparation and Storage
Store all kit components at room temperature.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for Rapid GST Inclusion Body Solubilization and Renaturation Kit assay kit
Background: Bacteria are widely used for His or GST tagged recombinant protein expression. GST fusion proteins in soluble form are purified from bacterial lysates by affinity chromatography using immobilized glutathione. However, recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels. It is not known exactly how they are formed, but it is thought that the protein within the inclusion body is partially or incorrectly folded. Once these inclusion bodies are formed, it is very difficult to solubilize them in a native, active conformation. The Rapid GST Inclusion Body Solubilization and Renaturation Kit is designed to retrieve expressed GST fusion protein in soluble form after lysis and extraction procedures. The detergent solubilization and neutralization reagents contained in the kit provides the most effective means for solubilizing and renaturing aggregated proteins without lengthy dialysis steps. The solubilization and neutralization steps only take 2 hrs (see Figure 1). The kit provides enough reagents for solubilizing and renaturing up to 5-10 liters of bacterial culture.
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References
1. Shin SI, GST Gene Fusion System Handbook, Amersham Biosciences, Code No. 18-1157-58.
1. Keller, D. et al. (2014). Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells. J. Cell Biol. 204:697-712.
2. Lalani, S. et al. (2013). MCTP2 is a Dosage-Sensitive Gene Required for Cardiac Outflow Tract Development. Hum. Mol. Genet. 10.1093/hmg/ddt283.
3. Sabbah, M. et al. (2011). CCN5, A Novel Transcriptional Repressor of Transforming Growth Factor-beta Signaling Pathway. Mol. Cell. Biol. 10.1128/MCB.01316-10.
1. Keller, D. et al. (2014). Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells. J. Cell Biol. 204:697-712.
2. Lalani, S. et al. (2013). MCTP2 is a Dosage-Sensitive Gene Required for Cardiac Outflow Tract Development. Hum. Mol. Genet. 10.1093/hmg/ddt283.
3. Sabbah, M. et al. (2011). CCN5, A Novel Transcriptional Repressor of Transforming Growth Factor-beta Signaling Pathway. Mol. Cell. Biol. 10.1128/MCB.01316-10.
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Product Notes
The Rapid GST Inclusion Body Solubilization and Renaturation Kit (Catalog #AAA168356) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Rapid GST Inclusion Body Solubilization and Renaturation Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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