Typical Testing Data/Standard Curve (for reference only)
(Fig. Protein Quantification Kit (Bradford Assay) standard curve)
Protein Assay Kit
Protein Quantification Kit (Bradford Assay)
Synonyms
Protein; Protein Quantification Kit (Bradford Assay); Coomassie; Protein assay kit
Detection Range
50-1000 ug/ml
Detection Method
Colorimetric
Limit of Detection
25 ug/ml
Features & Benefits
Almost immediate color development.
Unaffected by most chemical substances. The concentration of mercaptoethanol and dithiothreitol in the samples can reach 1mM and 5 mM respectively.
Working range from 50 to 1000 ug/ml.
Unaffected by most chemical substances. The concentration of mercaptoethanol and dithiothreitol in the samples can reach 1mM and 5 mM respectively.
Working range from 50 to 1000 ug/ml.
Usage Notes
Due to the influence of high concentration detergent, it is necessary to ensure that the concentration of SDS is lower than 0.1 %, Triton X-100 is lower than 0.1 % and Tween 20, 60, 80 is lower than 0.06 % in the sample.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig. Protein Quantification Kit (Bradford Assay) standard curve)
Typical Testing Data/Standard Curve (for reference only)
(Fig. Protein Quantification Kit (Bradford Assay) standard curve)
Related Product Information for Protein assay kit
Description: Protein Quantification Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in samples. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. When coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465nm to 595nm with a concomitant color change from brown to blue. The intensity of the blue complex is proportional to the amount of protein in the sample.
Background: The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250. The Coomassie Brilliant Blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The cationic (unbound) form is green/ red and has an absorption spectrum maximum historically held to be at 465 nm. The anionic bound form of the dye which is held together by hydrophobic and ionic interactions, has an absorption spectrum maximum historically held to be at 595 nm. The increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample.
Background: The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250. The Coomassie Brilliant Blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The cationic (unbound) form is green/ red and has an absorption spectrum maximum historically held to be at 465 nm. The anionic bound form of the dye which is held together by hydrophobic and ionic interactions, has an absorption spectrum maximum historically held to be at 595 nm. The increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample.
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Product Notes
The Protein (Catalog #AAA9711522) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protein, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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