Platinum Retroviral Expression System, Amphotropic Assay Kit
Platinum Retroviral Expression System, Amphotropic
Synonyms
Platinum Retroviral Expression System; Amphotropic; Amphotropic assay kit
Shipping Note
Product available only in the USA.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Product Note
Product is available for ACADEMIC, GOVERNMENT AND NON-PROFIT RESEARCH USE ONLY. For-profit institutions and other entities, please contact us for additional information.
Preparation and Storage
Store all plasmids at -20 degree C. For best results begin culture of Platinum cells upon receipt. If this is not possible, store at -80 degree C until first culture. Store subsequent cultured cells in liquid nitrogen.
Related Product Information for Platinum Retroviral Expression System, Amphotropic assay kit
Background: Retroviral gene transfer is a technique for efficiently introducing stable, heritable genetic material into the genome of any dividing cell type. With Moloney Murine Leukemia Virus (MMLV) - based vectors, transduction efficiencies of >90% are achievable for most mitotic cell types, and the copy number per cell can be easily controlled by varying the multiplicity of infection. Current retroviral gene transfer technology is based on the coordinated design of packaging cell lines and retroviral expression vectors. The packaging cell line usually contains the viral gag, pol, and env genes, necessary for particle formation and replication, these genes are stably integrated into the packaging cell genome. Retroviral expression vectors provide the packaging signal ?+, transcription and processing elements, and a target gene. Inserts of up to 6.5 kb can be efficiently packaged. Transfection of the retroviral vector into a packaging cell line produces high-titer, replication-incompetent virus (Table 1). The viral env gene, expressed by the packaging cell line, encodes the envelope protein, which determines the range of infectivity (tropism) of the packaged virus. Viral envelopes are classified according to the receptors used to enter host cells (Table 1). Ecotropic virus can recognize a receptor found on only mouse and rat cells. Amphotropic virus recognizes a receptor found on a broad range of mammalian cell types. Pantropic virus, such as retrovirus pseudo-typed with the envelope glycoprotein from the vesicular stomatitis virus (VSV-G), can infect both mammalian and non-mammalian cells. Unlike other viral envelope proteins, VSV-G mediates viral entry through lipid binding and plasma membrane fusion. However, stable expression of the VSV-G envelope protein is toxic, pantropic virus is usually produced by transiently cotransfecting a retroviral expression vector and pVSV-G into a pantropic packaging cell line expressing only the viral gag and pol genes. Conventional NIH-3T3 based retroviral packaging cell lines and Phoenix packaging cell lines have limited stability and produce low viral yields, mainly due to poor expression level of the retroviral structure proteins (gag, pol, env) in the packaging cells. The Platinum Packaging Cell Lines, potent retrovirus packaging cell lines based on the 293T cell line, were generated using novel packaging constructs with an EF1alpha promoter to ensure longer stability and high-yield retroviral structure protein expression (gag, pol, env). Platinum cells can be kept in good condition for at least 4 months in the presence of drug selection, and can produce retroviruses with an average titer of 1 x 107 infectious units/mL by transient transfection. In addition, replication competent retroviruses (RCR) are virtually nonexistent because only coding sequences of viral structural genes are used, avoiding any unnecessary retroviral sequences. Amphotropic Platinum Retrovirus Expression System provides a simple method to generate RCR-free amphotropic retrovirus at high titer. The kit includes Plat-A packaging cells, pMXs-Puro and pMXs-Neo retrovirus expression vectors and pMXs-GFP control vector.
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Product Notes
The Platinum Retroviral Expression System, Amphotropic (Catalog #AAA169402) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Platinum Retroviral Expression System, Amphotropic, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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