Nitric Oxide Synthase (NOS) Assay Kit | NOS assay kit
Nitric Oxide Synthase Assay Kit (NOS)
Gene Names
NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
Applications
Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
Synonyms
Nitric Oxide Synthase (NOS); Nitric Oxide Synthase Assay Kit (NOS); Nitric Oxide Synthase Assay; NOS assay kit
Sequence Length
1433
Applicable Applications for NOS assay kit
Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
Application Notes
1. Prepare samples and controls in 0.5 mL 1X Assay Buffer or buffer of choice at a cell density between 3-5 x 105 cells/mL. If samples were cultured in serum-containing medium, wash the samples prior to adding the DAF-2DA dye.
2. Prepare DAF-2DA staining solution by adding 56 uL DAF-2DA to 444 uL water. This is sufficient volume to stain 50 x 0.5 mL or 100 x 0.25 mL samples.
3. Pre-load samples with DAF-2DA staining solution by adding 10 uL into 490 uL cultured cells, or 5 uL into 245 uL cultured cells.
4. Incubate 1 hour at 37 degree C.
5. Wash samples at least once with 1X Assay Buffer to remove excess dye, and then resuspend in 1X Assay Buffer.
6. Treat cells with test compounds for desired period of time to induce NOS production. Keep cells protected from light.
7. Analyze with a flow cytometer, fluorescence plate reader, or fluorescence microscope. DAF-2DA excites at 488 nm and emits at 515 nm.
2. Prepare DAF-2DA staining solution by adding 56 uL DAF-2DA to 444 uL water. This is sufficient volume to stain 50 x 0.5 mL or 100 x 0.25 mL samples.
3. Pre-load samples with DAF-2DA staining solution by adding 10 uL into 490 uL cultured cells, or 5 uL into 245 uL cultured cells.
4. Incubate 1 hour at 37 degree C.
5. Wash samples at least once with 1X Assay Buffer to remove excess dye, and then resuspend in 1X Assay Buffer.
6. Treat cells with test compounds for desired period of time to induce NOS production. Keep cells protected from light.
7. Analyze with a flow cytometer, fluorescence plate reader, or fluorescence microscope. DAF-2DA excites at 488 nm and emits at 515 nm.
Sample
Cell culture
Excitation/Emission
488 nm / 515 nm
Preparation and Storage
Store at 2-8 degree C. Protect DAF-2DA dye from light.
Related Product Information for NOS assay kit
Description: Nitric Oxide Synthase Assay provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This kit assesses the overall intracellular levels of free nitric oxide and NOS using a Diaminofluorescein-2 Diacetate (DAF-2DA) dye. Results can be analyzed using a flow cytometer, fluorescence plate reader, or fluorescence microscope.
Background: Nitric oxide synthases are a family of enzymes capable of catalyzing the production of nitric oxide (NO). NO is an important molecule that is involved in regulating a variety of cellular processes such as angiogenesis, peristalsis, and the immune response to invading pathogens. Reactive nitrogen species are a family of antimicrobial molecules derived from NO and superoxide through the enzymatic activities of nitric oxide synthase (NOS).
Nitric Oxide Synthase Assay provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This kit assesses the overall intracellular levels of free nitric oxide and NOS using a Diaminofluorescein-2 Diacetate (DAF-2DA) dye. Results can be analyzed using a flow cytometer, fluorescence plate reader, or fluorescence microscope.
Background: Nitric oxide synthases are a family of enzymes capable of catalyzing the production of nitric oxide (NO). NO is an important molecule that is involved in regulating a variety of cellular processes such as angiogenesis, peristalsis, and the immune response to invading pathogens. Reactive nitrogen species are a family of antimicrobial molecules derived from NO and superoxide through the enzymatic activities of nitric oxide synthase (NOS).
Nitric Oxide Synthase Assay provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This kit assesses the overall intracellular levels of free nitric oxide and NOS using a Diaminofluorescein-2 Diacetate (DAF-2DA) dye. Results can be analyzed using a flow cytometer, fluorescence plate reader, or fluorescence microscope.
Product Categories/Family for NOS assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
nitric oxide synthase
NCBI Official Synonym Full Names
nitric oxide synthase 1
NCBI Official Symbol
NOS1
NCBI Official Synonym Symbols
NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
NCBI Protein Information
nitric oxide synthase, brain
Protein Family
NCBI Description
The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]
Research Articles on NOS
Similar Products
Product Notes
The NOS (Catalog #AAA258378) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nitric Oxide Synthase (NOS) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader. 1. Prepare samples and controls in 0.5 mL 1X Assay Buffer or buffer of choice at a cell density between 3-5 x 105 cells/mL. If samples were cultured in serum-containing medium, wash the samples prior to adding the DAF-2DA dye. 2. Prepare DAF-2DA staining solution by adding 56 uL DAF-2DA to 444 uL water. This is sufficient volume to stain 50 x 0.5 mL or 100 x 0.25 mL samples. 3. Pre-load samples with DAF-2DA staining solution by adding 10 uL into 490 uL cultured cells, or 5 uL into 245 uL cultured cells. 4. Incubate 1 hour at 37 degree C. 5. Wash samples at least once with 1X Assay Buffer to remove excess dye, and then resuspend in 1X Assay Buffer. 6. Treat cells with test compounds for desired period of time to induce NOS production. Keep cells protected from light. 7. Analyze with a flow cytometer, fluorescence plate reader, or fluorescence microscope. DAF-2DA excites at 488 nm and emits at 515 nm. Researchers should empirically determine the suitability of the NOS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nitric Oxide Synthase (NOS), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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