Typical Testing Data/Standard Curve (for reference only)
ApoBrdU Red Assay Kit
ApoBrdU Red DNA Fragmentation Kit
Synonyms
ApoBrdU Red; ApoBrdU Red DNA Fragmentation Kit; ApoBrdU Red assay kit
Samples
Tissue sections and fixed cell preparations
Features & Benefits
Simple procedure; takes ~ 3 hours
Fast and convenient
The assay is sensitive and stable
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Fast and convenient
The assay is sensitive and stable
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ApoBrdU Red assay kit
Product Information: Apo-BrdU-RedTM In Situ DNA Fragmentation Assay Kit: Convenient & Reliable Br-dUTP based Assay to Detect DNA Fragmentation by Fluorescence Microscopy or Flow Cytometry. Includes both Positive and Negative Control Cells. Suitable for Studying Apoptosis with GFP Transfected Cells. 60 Assays.
Background: Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. MyBioSource's Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.
Summary: Detection method- Flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD).
Species reactivity- Mammalian
Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control cells for convenient detection of DNA fragmentation
Background: Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. MyBioSource's Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.
Summary: Detection method- Flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD).
Species reactivity- Mammalian
Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control cells for convenient detection of DNA fragmentation
Product Categories/Family for ApoBrdU Red assay kit
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Product Notes
The ApoBrdU Red (Catalog #AAA841582) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ApoBrdU Red, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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