Antithrombin III Assay Kit | SERPINC1 assay kit

Antithrombin III Antibody Pair

Cat. Num. AAA539763

• Gene Names: SERPINC1; AT3; AT3D; ATIII; THPH7
• Applications: ELISA
• Synonyms: Antithrombin III; Antithrombin III Antibody Pair; Anti Antithrombin III Pair; SERPINC1 assay kit

• Specificity: Human
• Sequence Length: 464

• Applicable Applications for SERPINC1 assay kit: ELISA (EIA)
• Preparation and Storage: Store at 4 degree C

Related Product Information for SERPINC1 assay kit

Principle of the Assay: Affinity-purified antibody to ATIII is coated onto the wells of a micro titre plate. The plates are washed and plasma or other fluids containing ATIII are applied. The coated antibody will capture the ATIII in the sample. After washing the plate to remove unbound material, a peroxidase conjugated second antibody to ATIII is added to the plate to bind to the captured ATIII. After washing the plate to remove unbound conjugated antibody, the peroxidase activity is expressed by incubation with o-phenylenediamine (OPD). After a fixed development time the reaction is quenched with the addition of H2SO4 and the colour produced is quantified using a microplate reader. The colour generated is proportional to the concentration of ATIII present in the sample.!!Background/Introduction: Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200 ug/ml (~3.5 uM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg-Ser and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated factor X is increased 1000- fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59 kDa. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96 kDa.

Product Categories/Family for SERPINC1 assay kit

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4502261
• NCBI GeneID: 462
• NCBI Accession #: NP_000479.1
• NCBI GenBank Nucleotide #: NM_000488.3
• Molecular Weight: 52,602 Da
• NCBI Official Full Name: antithrombin-III
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade C (antithrombin), member 1
• NCBI Official Symbol: SERPINC1
• NCBI Official Synonym Symbols: AT3; AT3D; ATIII; THPH7
• NCBI Protein Information: antithrombin-III
• UniProt Protein Name: Antithrombin-III
• Protein Family: Antithrombin
• UniProt Gene Name: SERPINC1
• UniProt Synonym Gene Names: AT3; ATIII
• UniProt Entry Name: ANT3_HUMAN

NCBI Description

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]

Uniprot Description

SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; protein binding; protease binding

Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient

Disease: Antithrombin Iii Deficiency