Human Argininosuccinate synthase ELISA Kit | ASS1 elisa kit

Human Argininosuccinate synthase ELISA Kit

Cat. Num. AAA760309

• Gene Names: ASS1; CN; ASS
• Reactivity: Human
• Synonyms: Argininosuccinate synthase; Human Argininosuccinate synthase ELISA Kit; ASS1/Citrulline--aspartate ligase/ASS; ASS1 elisa kit

• Reactivity: Human
• Sequence Length: 412

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ASS1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology anti-Human ASS1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ASS1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ASS1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ASS1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 27806925
• NCBI GeneID: 280726
• NCBI Accession #: NP_776317.1
• NCBI GenBank Nucleotide #: NM_173892.4
• Molecular Weight: 46,417 Da
• NCBI Official Full Name: argininosuccinate synthase
• NCBI Official Symbol: ASS1
• NCBI Official Synonym Symbols: CN; ASS
• NCBI Protein Information: argininosuccinate synthase
• UniProt Protein Name: Argininosuccinate synthase
• Protein Family: Argininosuccinate synthase
• UniProt Gene Name: ASS1
• UniProt Synonym Gene Names: ASS
• UniProt Entry Name: ASSY_BOVIN

Uniprot Description

ASS1: Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1). Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood. Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Protein type: Mitochondrial; Ligase; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; Endoplasmic reticulum; EC 6.3.4.5

Cellular Component: cytoplasm

Molecular Function: amino acid binding; argininosuccinate synthase activity; ATP binding; identical protein binding

Biological Process: arginine biosynthetic process; argininosuccinate metabolic process; aspartate metabolic process; citrulline metabolic process; positive regulation of nitric oxide biosynthetic process; urea cycle