SDS-Page
ART4 recombinant protein
ART4, 47-285aa, Human, His-tag, Baculovirus
Gene Names
ART4; DO; DOK1; ARTC4; CD297
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
ART4; 47-285aa; Human; His-tag; Baculovirus; Ecto-ADP-ribosyltransferase 4 isoform 1; ARTC4; CD297; DO; DOK1; ART4 recombinant protein
Host
Baculovirus
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
ADPSEVAIKI DFDFAPGSFD DQYQGCSKQV MEKLTQGDYF TKDIEAQKNY FRMWQKAHLA WLNQGKVLPQ NMTTTHAVAI LFYTLNSNVH SDFTRAMASV ARTPQQYERS FHFKYLHYYL TSAIQLLRKD SIMENGTLCY EVHYRTKDVH FNAYTGATIR FGQFLSTSLL KEEAQEFGNQ TLFTIFTCLG APVQYFSLKK EVLIPPYELF KVINMSYHPR GDWLQLRSTG NLSTYNCQLL KAHHHHHH
Sequence Length
314
Applicable Applications for ART4 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for ART4 recombinant protein
ART4, also known as ecto-ADP-ribosyltransferase 4, is a glycosylphosphatidylinositol (GPI)-linked membrane protein carrying Dombrock blood group antigens. It is developmentally regulated during erythroid differentiation and occurs at highest levels in the fetal liver. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Recombinant human ART4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for ART4 recombinant protein
References
Grodecka M., et al. (2012) Immunohematology. 28:124-129.; Gubin AN., et al. (2000) Blood. 96:2621-2627.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28.8kDa (248aa) 28-40kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
ecto-ADP-ribosyltransferase 4 isoform 1
NCBI Official Synonym Full Names
ADP-ribosyltransferase 4 (Dombrock blood group)
NCBI Official Symbol
ART4
NCBI Official Synonym Symbols
DO; DOK1; ARTC4; CD297
NCBI Protein Information
ecto-ADP-ribosyltransferase 4
UniProt Protein Name
Ecto-ADP-ribosyltransferase 4
Protein Family
UniProt Gene Name
ART4
UniProt Synonym Gene Names
DO; DOK1; ARTC4
NCBI Description
This gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known. [provided by RefSeq, Jul 2008]
Research Articles on ART4
Similar Products
Product Notes
The ART4 art4 (Catalog #AAA206781) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ART4 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ART4 art4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPSEVAIKI DFDFAPGSFD DQYQGCSKQV MEKLTQGDYF TKDIEAQKNY FRMWQKAHLA WLNQGKVLPQ NMTTTHAVAI LFYTLNSNVH SDFTRAMASV ARTPQQYERS FHFKYLHYYL TSAIQLLRKD SIMENGTLCY EVHYRTKDVH FNAYTGATIR FGQFLSTSLL KEEAQEFGNQ TLFTIFTCLG APVQYFSLKK EVLIPPYELF KVINMSYHPR GDWLQLRSTG NLSTYNCQLL KAHHHHHH. It is sometimes possible for the material contained within the vial of "ART4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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