Aryl Hydrocarbon Receptor Nuclear Translocator 2 Recombinant Protein | ARNT2 recombinant protein

Recombinant Aryl Hydrocarbon Receptor Nuclear Translocator 2 (ARNT2)

Cat. Num. AAA2033119

• Gene Names: ARNT2; WEDAS; bHLHe1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Aryl Hydrocarbon Receptor Nuclear Translocator 2; Recombinant Aryl Hydrocarbon Receptor Nuclear Translocator 2 (ARNT2); bHLHe1; Class E basic helix-loop-helix protein 1; ARNT2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MATPAAVNPP EMASDIPGSV TLPVAPMAAT GQVRMAGAMP ARGGKRRSGM DFDDEDGEGP SKFSRENHSE IERRRRNKMT QYITELSDMV PTCSALARKP DKLTILRMAV SHMKSMRGTG NKSTDGAYKP SFLTEQELKH LILEAADGFL FVVAAETGRV IYVSDSVTPV LNQPQSEWFG STLYEQVHPD DVEKLREQLC TSENSMTGR
• Sequence Length: 717

• Applicable Applications for ARNT2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 26.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Arg209
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 68303555
• NCBI GeneID: 9915
• NCBI Accession #: NP_055677.3
• NCBI GenBank Nucleotide #: NM_014862.3
• Molecular Weight: 77,612 Da
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator 2
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator 2
• NCBI Official Symbol: ARNT2
• NCBI Official Synonym Symbols: WEDAS; bHLHe1
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator 2
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator 2
• Protein Family: Aryl hydrocarbon receptor nuclear translocator
• UniProt Gene Name: ARNT2
• UniProt Synonym Gene Names: BHLHE1; KIAA0307; ARNT protein 2; bHLHe1
• UniProt Entry Name: ARNT2_HUMAN

NCBI Description

This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting additional roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively. [provided by RefSeq, Dec 2013]

Uniprot Description

ARNT2: Specifically recognizes the xenobiotic response element (XRE). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: cytoplasm; nucleus; transcription factor complex

Molecular Function: aryl hydrocarbon receptor binding; protein heterodimerization activity; transcription factor activity

Biological Process: brain development; central nervous system development; in utero embryonic development; negative regulation of apoptosis; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to estradiol stimulus; response to hypoxia; transcription from RNA polymerase II promoter

Disease: Webb-dattani Syndrome