Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (ARFGEF1) Recombinant Protein | ARFGEF1 recombinant protein

Recombinant Bovine Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (ARFGEF1) , partial

• Gene Names: ARFGEF1; BIG1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (ARFGEF1); Recombinant Bovine Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (ARFGEF1); partial; ARFGEF1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,849

• Species: Bovine
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for ARFGEF1 recombinant protein

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. This protein is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.

NCBI and Uniprot Product Information

• NCBI GI #: 27806733
• NCBI GeneID: 281022
• NCBI Accession #: NP_776422.1
• NCBI GenBank Nucleotide #: NM_173997.2
• UniProt Accession #: O46382
• Molecular Weight: 208,712 Da
• NCBI Official Full Name: brefeldin A-inhibited guanine nucleotide-exchange protein 1
• NCBI Official Synonym Full Names: ADP ribosylation factor guanine nucleotide exchange factor 1
• NCBI Official Symbol: ARFGEF1
• NCBI Official Synonym Symbols: BIG1
• NCBI Protein Information: brefeldin A-inhibited guanine nucleotide-exchange protein 1
• UniProt Protein Name: Brefeldin A-inhibited guanine nucleotide-exchange protein 1
• UniProt Gene Name: ARFGEF1
• UniProt Synonym Gene Names: ARFGEP1; BIG1; Brefeldin A-inhibited GEP 1

Uniprot Description

Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined ().