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Gene Sequencing (extract)

Aquaporin 4 Recombinant Protein | AQP4 recombinant protein

Recombinant Aquaporin 4 (AQP4)

Gene Names
AQP4; MIWC; HMIWC2
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Aquaporin 4; Recombinant Aquaporin 4 (AQP4); AQP4 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 300ug/mL (varies by lot)
Sequence
CDS KRTDVTGSIA LAIGFSVAIG HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG AVLAGGLYEY VFCPDVEFKR RFKEAFSKAA QQTKGSYMEV EDNRSQVETD DLILKPGVVHVIDVDRGEEK KGKDQSG
Applicable Applications for AQP4 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Cys178~Gly317
Tags
N-terminal His and GST Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
5.6
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Gene Sequencing (extract)

Gene Sequencing (extract)

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
361
Molecular Weight
Predicted Molecular Mass: 45.4kDa
Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
Aquaporin 4
NCBI Official Synonym Full Names
aquaporin 4
NCBI Official Symbol
AQP4
NCBI Official Synonym Symbols
MIWC; HMIWC2
NCBI Protein Information
aquaporin-4
UniProt Protein Name
Aquaporin-4
Protein Family
UniProt Gene Name
AQP4
UniProt Synonym Gene Names
AQP-4; MIWC
UniProt Entry Name
AQP4_HUMAN

NCBI Description

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Transporter, aquaporin family; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 18q11.2-q12.1

Cellular Component: protein complex; integral to plasma membrane; basolateral plasma membrane; cytoplasm; T-tubule; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: water transporter activity; water channel activity; porin activity; glycerol channel activity

Biological Process: glycerol transport; cellular water homeostasis; carbon dioxide transport; water transport; response to glucocorticoid stimulus; female pregnancy; response to radiation; sensory perception of sound; hyperosmotic salinity response; transport; renal water homeostasis; multicellular organismal water homeostasis; transmembrane transport; protein homooligomerization

Research Articles on AQP4

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Product Notes

The AQP4 aqp4 (Catalog #AAA2030012) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Aquaporin 4 (AQP4) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Aquaporin 4 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the AQP4 aqp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CDS KRTDVTGSIA LAIGFSVAIG HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG AVLAGGLYEY VFCPDVEFKR RFKEAFSKAA QQTKGSYMEV EDNRSQVETD DLILKPGVVH VIDVDRGEEK KGKDQSG. It is sometimes possible for the material contained within the vial of "Aquaporin 4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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