AB1-42 recombinant protein

Recombinant Mature Human AB1-42 Protein

Cat. Num. AAA355847

• Gene Names: APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
• Purity: >85% by SDS-PAGE
• Synonyms: AB1-42; Recombinant Mature Human AB1-42 Protein; AB1-42 Protein; Amyloid beta 1-42; Beta Amyloid(1-42); beta Amyloid 1-42; beta-Amyloid 1-42; Amyloid 1-42; A4; ABETA; ABPP; Alzheimers Disease Amyloid Protein; Amyloid B; Amyloid Beta A4 Protein Precursor; Amyloid Beta; Amyloid of Aging and Alzheimer Disease; B Amyloid; Beta APP; Cerebral Vascular Amyloid Peptide; Beta-amyloid protein 42; AB1-42 recombinant protein

• Host: E Coli BL21
• Purity/Purification: >85% by SDS-PAGE
• Sequence Length: 770

• Storage Buffer: Contains 0.01M PBS, pH 7.5.
• Preparation and Storage: Ship at 4 degree C. Upon receipt, aliquot and store at -20 degree C for long term.; Avoid repeated freeze and thaw cycles.

Related Product Information for AB1-42 recombinant protein

Amyloid beta (Abeta or Abeta) denotes peptides of 36-43 amino acids that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. Oligomeric amyloid-beta 1-42 (Abeta-42) peptides are considered to be the most toxic species connected to the occurrence of Alzheimer's disease. Unstructured oligomeric Abeta-42 interacted with membrane bilayers of giant unilamellar vesicles (GUV) and neuronal model cells, facilitated cellular uptake of Abeta-42, and inhibition of mitochondrial activity. Elevated concentrations of glucose within the range observed in diabetic individuals (10 mM) facilitate the formation of membrane-active Abeta-42 oligomers.

References

1. Kedia N, Almisry M, Bieschke J. Glucose directs amyloid-beta into membrane-active oligomers. Phys Chem Chem Phys. 2017 Jul 21;19(27):18036-18046.

NCBI and Uniprot Product Information

• NCBI GI #: 4502167
• NCBI GeneID: 351
• NCBI Accession #: NP_000475.1
• NCBI GenBank Nucleotide #: NM_000484.3
• UniProt Accession #: P05067; P09000; P78438; Q13764; Q13778; Q13793; Q16011; B2R5V1; B4DII8; D3DSD1; D3DSD2; D3DSD3
• Molecular Weight: 84,521 Da
• NCBI Official Full Name: amyloid-beta A4 protein isoform a
• NCBI Official Synonym Full Names: amyloid beta precursor protein
• NCBI Official Symbol: APP
• NCBI Official Synonym Symbols: AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
• NCBI Protein Information: amyloid-beta A4 protein
• UniProt Protein Name: Amyloid-beta A4 protein
• UniProt Gene Name: APP
• UniProt Synonym Gene Names: A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; Beta-CTF; Abeta42; Abeta40; Alpha-CTF; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)

NCBI Description

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Uniprot Description

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu2+ ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.