Bovine Amyloid precursor protein fragment C99 ELISA Kit | APP-C99 elisa kit

Bovine Amyloid precursor protein fragment C99 ELISA Kit

Cat. Num. AAA745035

• Reactivity: Bovine
• Synonyms: Amyloid precursor protein fragment C99; Bovine Amyloid precursor protein fragment C99 ELISA Kit; APP-C99 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This APP-C99 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine APP-C99. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APP-C99 elisa kit

Principle of the Assay: APP-C99 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APP-C99 antibody and an APP-C99-HRP conjugate. The assay sample and buffer are incubated together with APP-C99-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APP-C99 concentration since APP-C99 from samples and APP-C99-HRP conjugate compete for the anti-APP-C99 antibody binding site. Since the number of sites is limited, as more sites are occupied by APP-C99 from the sample, fewer sites are left to bind APP-C99-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APP-C99 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APP-C99 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 236721
• NCBI Official Full Name: amyloid protein, partial

NCBI Description

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]