Apolipoprotein M Recombinant Protein | APOM recombinant protein

Recombinant Apolipoprotein M (APOM)

Cat. Num. AAA2031169

• Gene Names: APOM; G3a; NG20; apo-M; HSPC336
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Apolipoprotein M; Recombinant Apolipoprotein M (APOM); APOM recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; QCPEYSQLT TQGVDGKEFP EPHLGRWYFIAGAAPTKEDL ATFDPVDNIV FNMAADSVPL QLRLRATIRT KNGLCVPRKWIYHLTEGSTD LRTEGRPDMK TKLFSSSCPG GIMLKETGQG YQRFLLYNRSPHPPEKCVEE FQSLTSCLDS KAFLLTPRNQ EACELSNN
• Sequence Length: 188

• Applicable Applications for APOM recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 22.5kDa
• Accurate Molecular Mass (KD): 23kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Equus caballus; Equine (Horse)
• Fragment: Gln22~Asn188
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 18089092
• NCBI GeneID: 55937
• Molecular Weight: 13,051 Da
• NCBI Official Full Name: Apolipoprotein M
• NCBI Official Synonym Full Names: apolipoprotein M
• NCBI Official Symbol: APOM
• NCBI Official Synonym Symbols: G3a; NG20; apo-M; HSPC336
• NCBI Protein Information: apolipoprotein M
• UniProt Protein Name: Apolipoprotein M
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOM
• UniProt Synonym Gene Names: G3A; NG20; Apo-M; ApoM
• UniProt Entry Name: APOM_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: integral to plasma membrane

Molecular Function: antioxidant activity; lipid transporter activity; phospholipid binding

Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; cholesterol efflux; lipoprotein metabolic process