APOH recombinant protein

APOH, 20-345aa, Human, His-tag, Baculovirus

Cat. Num. AAA206498

• Gene Names: APOH; BG; B2G1; B2GP1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: APOH; 20-345aa; Human; His-tag; Baculovirus; Beta-2-glycoprotein 1; BG; B2G1; B2GP1; Apolipoprotein H; APOH recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPGRTCPKP DDLPFSTVVP LKTFYEPGEE ITYSCKPGYV SRGGMRKFIC PLTGLWPINT LKCTPRVCPF AGILENGAVR YTTFEYPNTI SFSCNTGFYL NGADSAKCTE EGKWSPELPV CAPIICPPPS IPTFATLRVY KPSAGNNSLY RDTAVFECLP QHAMFGNDTI TCTTHGNWTK LPECREVKCP FPSRPDNGFV NYPAKPTLYY KDKATFGCHD GYSLDGPEEI ECTKLGNWSA MPSCKASCKV PVKKATVVYQ GERVKIQEKF KNGMLHGDKV SFFCKNKEKK CSYTEDAQCI DGTIEVPKCF KEHSSLAFWK TDASDVKPCH HHHHH
• Sequence Length: 345

• Applicable Applications for APOH recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for APOH recombinant protein

APOH, also known as Beta-2-glycoprotein 1, is a glycosylated member of the complement control superfamily of proteins. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. This protein may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. It has a complex involvement in agglutination, it appears to alter Adenosine diphosphate (ADP) mediated agglutination of platelets. Recombinant human APOH protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for APOH recombinant protein

Cardiovascular

References

About F. et al., (2015) PLoS One. 10:e0145105. ; Laird ME. et al., (2014) J Hepatol. 61:770-776.

NCBI and Uniprot Product Information

• NCBI GI #: 153266841
• NCBI GeneID: 350
• NCBI Accession #: NP_000033
• NCBI GenBank Nucleotide #: NM_000042.2
• UniProt Accession #: P02749; Q9UCN7; B2R9M3
• Molecular Weight: 37.3kDa (335aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: beta-2-glycoprotein 1
• NCBI Official Synonym Full Names: apolipoprotein H
• NCBI Official Symbol: APOH
• NCBI Official Synonym Symbols: BG; B2G1; B2GP1
• NCBI Protein Information: beta-2-glycoprotein 1
• UniProt Protein Name: Beta-2-glycoprotein 1
• Protein Family: Beta-2-glycoprotein
• UniProt Gene Name: APOH
• UniProt Synonym Gene Names: B2G1; Apo-H; B2GPI; Beta(2)GPI

NCBI Description

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.