Human Apolipoprotein F ELISA Kit | APOF elisa kit

Human Apolipoprotein F ELISA Kit

Cat. Num. AAA38598

• Gene Names: Apof; AI255964
• Reactivity: Human
• Synonyms: Apolipoprotein F; Human Apolipoprotein F ELISA Kit; APOF elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human APOF and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 6.25mug/ml-200mug/ml.
• Sensitivity: 1.0mug/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated APOF concentrations in Human serum, plasma and other body fluids. Using Purified Human APOF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOF and APOF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for APOF elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated APOF concentrations in Human serum, plasma and other body fluids. Using Purified Human APOF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOF and APOF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19527216
• NCBI GeneID: 103161
• NCBI Accession #: NP_598758.1
• NCBI GenBank Nucleotide #: NM_133997.2
• UniProt Accession #: Q91V80
• Molecular Weight: 34,359 Da
• NCBI Official Full Name: apolipoprotein F
• NCBI Official Synonym Full Names: apolipoprotein F
• NCBI Official Symbol: Apof
• NCBI Official Synonym Symbols: AI255964
• NCBI Protein Information: apolipoprotein F; LVIF; apo-F; leukemia virus-inactivating factor
• UniProt Protein Name: Apolipoprotein F
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apof
• UniProt Synonym Gene Names: Lvif; Apo-F; LVIF
• UniProt Entry Name: APOF_MOUSE

Uniprot Description

APOF: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular region

Biological Process: steroid metabolic process; cholesterol metabolic process; triacylglycerol metabolic process; transport; cholesterol efflux; lipid metabolic process; lipid transport