APOBEC3B recombinant protein

Recombinant Human APOBEC3B protein

Cat. Num. AAA1562524

• Gene Names: APOBEC3B; A3B; ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: APOBEC3B; Recombinant Human APOBEC3B protein; DNA dC->dU-editing enzyme APOBEC-3B; Short name:A3B; Phorbolin-1-related protein; Phorbolin-2/3; APOBEC3B recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human APOBEC3B (Met1-Arg190) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for APOBEC3B recombinant protein

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.

Product Categories/Family for APOBEC3B recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 393715117
• NCBI GeneID: 9582
• NCBI Accession #: NP_004891
• NCBI GenBank Nucleotide #: NM_004900.4
• UniProt Accession #: Q9UH17; O95618; Q20WL1; Q5IFJ4; Q7Z2N3; Q7Z6D6; Q9UE74; B0QYD2
• Molecular Weight: Predicted: 42 kDa; ; Observed: 48 kDa
• NCBI Official Full Name: DNA dC->dU-editing enzyme APOBEC-3B isoform a
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
• NCBI Official Symbol: APOBEC3B
• NCBI Official Synonym Symbols: A3B; ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3B; phorbolin 2; phorbolin 3; phorbolin-2/3; cytidine deaminase; phorbolin-1-related protein; probable DNA dC->dU-editing enzyme APOBEC-3B; apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3B
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3B
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3B
• UniProt Synonym Gene Names: A3B
• UniProt Entry Name: ABC3B_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

APOBEC3B: a DNA cytidine deaminase involved in foreign DNA clearance. Acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Homodimer. Interacts with APOBEC3G. Does not interact with APOBEC1. Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues. Phorbol 12-myristate 13-acetate (PMA) induces overexpression in keratinocytes. Up-regulated by IFN-alpha. Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; RNA-binding; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: nucleus

Molecular Function: zinc ion binding; deoxycytidine deaminase activity

Biological Process: metabolic process; innate immune response; defense response to virus