SDS-Page
(Recombinant APOBEC3A (A3A) SDS-PAGE gel Protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 23 kDa)
APOBEC3A (A3A) Recombinant Protein | APOBEC3 recombinant protein
Recombinant APOBEC3A (A3A) protein
Synonyms
APOBEC3A (A3A); Recombinant APOBEC3A (A3A) protein; Apolipoprotein B mRNA editing enzyme; catalytic polypeptide-like 3A; APOBEC3 recombinant protein
Host
E Coli
Form/Format
Recombinant APOBEC3A is supplied in 50mM Tris, pH7.4, 50mM NaCl, 10% glycerol, 1mM beta-mercaptoethanol, and 0.01% Tween-20.
Protein Species
Human
Notes
Recombinant APOBEC3A is suitable for use in bisulfite-free whole genome sequencing of 5-hydroxymethylcytosine (e.g., ACE-Seq), cytosine deamination, enzyme kinetics, inhibitor screening and selectively profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant APOBEC3A (A3A) SDS-PAGE gel Protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 23 kDa)
SDS-Page
(Recombinant APOBEC3A (A3A) SDS-PAGE gel Protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 23 kDa)
Testing Data
(HPLC-based DNA cleavage assay for APOBEC3A activity. A single-stranded DNA oligonucleotide bearing one deoxycytidine is incubated with recombinant APOBEC3A, leading to deamination of deoxycytidine, a dC dU conversion. Incubation of dU bearing oligonucleotide with uracil-DNA glycosylase creates an abasic site that is then highly susceptible to base-mediated strand cleavage. (A.) Intact, deoxycytidine-containing substrate oligonucleotide with an HPLC retention time of 9 minutes. (B.) APOBEC3A-deaminated and base-cleaved ssDNA oligo. Cleavage products have retention times of 8.1 and 8.4 minutes.)
Testing Data
(HPLC-based DNA cleavage assay for APOBEC3A activity. A single-stranded DNA oligonucleotide bearing one deoxycytidine is incubated with recombinant APOBEC3A, leading to deamination of deoxycytidine, a dC dU conversion. Incubation of dU bearing oligonucleotide with uracil-DNA glycosylase creates an abasic site that is then highly susceptible to base-mediated strand cleavage. (A.) Intact, deoxycytidine-containing substrate oligonucleotide with an HPLC retention time of 9 minutes. (B.) APOBEC3A-deaminated and base-cleaved ssDNA oligo. Cleavage products have retention times of 8.1 and 8.4 minutes.)
Related Product Information for APOBEC3 recombinant protein
Short Description: Recombinant APOBEC3A was expressed in E Coli as the full-length protein (accession number NP_663745) according to the protocol detailed in Schutksy, et al., Nucleic Acids Res. 2017 Jul 27. The molecular weight of rAPOBEC3A is 23 kDa. Recombinant APOBEC3A is suitable for use in bisulfite-free whole genome sequencing of 5-hydroxymethylcytosine (e.g., ACE-Seq), cytosine deamination, enzyme kinetics, inhibitor screening and selectively profiling.
Background: Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, also known as APOBEC3A, or A3A is a gene of the APOBEC3 family found in humans, non-human primates, and some other mammals. It is a single-domain DNA cytidine deaminase with antiviral effects. While other members of the family such as APOBEC3G are believed to act by editing ssDNA by removing an amino group from cytosine in DNA, introducing a cytosine to uracil change which can ultimately lead to a cytosine to thymine mutation, one study suggests that APOBEC3A can inhibit parvoviruses by another mechanism. The cellular function of APOBEC3A is likely to be the destruction of foreign DNA through extensive deamination of cytosine.
Background: Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, also known as APOBEC3A, or A3A is a gene of the APOBEC3 family found in humans, non-human primates, and some other mammals. It is a single-domain DNA cytidine deaminase with antiviral effects. While other members of the family such as APOBEC3G are believed to act by editing ssDNA by removing an amino group from cytosine in DNA, introducing a cytosine to uracil change which can ultimately lead to a cytosine to thymine mutation, one study suggests that APOBEC3A can inhibit parvoviruses by another mechanism. The cellular function of APOBEC3A is likely to be the destruction of foreign DNA through extensive deamination of cytosine.
Product Categories/Family for APOBEC3 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of rAPOBEC3A is 23kDa
Protein Family
Similar Products
Product Notes
The APOBEC3 (Catalog #AAA389331) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "APOBEC3A (A3A), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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