Apolipoprotein A-V Recombinant Protein | APOA5 recombinant protein

Recombinant Human Apolipoprotein A-V, HEK

Cat. Num. AAA144957

• Gene Names: APOA5; RAP3; APOAV
• Purity: Greater than 70% as determined by SDS-PAGE.
• Synonyms: Apolipoprotein A-V; Recombinant Human Apolipoprotein A-V; HEK; APOA5 Human; Apolipoprotein A-V Human Recombinant; Apo-AV; ApoA-V; Apolipoprotein A5; Regeneration-associated protein 3; APOA5; RAP3; APOAV; APOA5 HEK; APOA5 recombinant protein

• Host: HEK293
• Purity/Purification: Greater than 70% as determined by SDS-PAGE.
• Form/Format: APOA5 Human was filtered (0.4 um) and lyophilized from 0.5 mg/ml supplied in 20mM TRIS and 50mM NaCl, pH 7.5
• Sequence: RKGFWDYFSQ TSGDKGRVEQ IHQQKMAREP ATLKDSLEQD LNNMNKFLEK LRPLSGSEAP RLPQDPVGMR RQLQEELEEV KARLQPYMAE AHELVGWNLE GLRQQLKPYT MDLMEQVALR VQELQEQLRV VGEDTKAQLL GGVDEAWALL QGLQSRVVHH TGRFKELFHP YAESLVSGIG RHVQELHRSV APHAPASPAR LSRCVQVLSR KLTLKAKALH ARIQQNLDQL REELSRAFAG TGTEEGAGPD PQMLSEEVRQ RLQAFRQDTY LQIAAFTRAI DQETEEVQQQ LAPPPPGHSA FAPEFQQTDS GKVLSKLQAR LDDLWEDITH SLHDQGHSHL GDPAAADYKD DDDK
• Sequence Length: 366

• Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
• Preparation and Storage: Store lyophilized APOA5 Human at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted APOA5 Human can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for APOA5 recombinant protein

APOA5 Human Recombinant DYKDDDDK-Tagged Fusion Protein is 40.1 kDa protein containing 354 amino acid residues of the APOA5 Human and 11 additional amino acid residues of DYKDDDDK Tag.

Product Categories/Family for APOA5 recombinant protein

Cytokines and Growth Factors; Cytokines; Apolipoprotein

NCBI and Uniprot Product Information

• NCBI GI #: 262231737
• NCBI GeneID: 116519
• NCBI Accession #: NP_001160070.1
• NCBI GenBank Nucleotide #: NM_001166598.1
• UniProt Accession #: Q6Q788; Q3MIK6; Q6UWK9; Q9UBJ3; B0YIV9
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A-V
• NCBI Official Symbol: APOA5
• NCBI Official Synonym Symbols: RAP3; APOAV
• NCBI Protein Information: apolipoprotein A-V; apo-AV; apolipoprotein A5; regeneration-associated protein 3
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA5
• UniProt Synonym Gene Names: RAP3; Apo-AV; ApoA-V
• UniProt Entry Name: APOA5_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; extracellular region

Molecular Function: heparin binding; enzyme binding; low-density lipoprotein receptor binding; cholesterol transporter activity; enzyme activator activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; organ regeneration; response to hormone stimulus; neurite regeneration; lipoprotein metabolic process; positive regulation of lipid catabolic process; cholesterol efflux; cellular lipid metabolic process; lipid transport; cholesterol biosynthetic process; positive regulation of receptor-mediated endocytosis; phosphatidylcholine metabolic process; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid efflux; regulation of cholesterol absorption; acylglycerol homeostasis; tissue regeneration; triacylglycerol catabolic process; positive regulation of lipoprotein lipase activity

Disease: Hypertriglyceridemia, Familial; Hyperlipoproteinemia, Type V