Amyloid Beta Precursor Protein Binding Protein B1 (APBB1) Recombinant Protein | APBB1 recombinant protein

Recombinant Amyloid Beta Precursor Protein Binding Protein B1 (APBB1)

Cat. Num. AAA2086798

• Gene Names: APBB1; RIR; FE65; MGC:9072
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Amyloid Beta Precursor Protein Binding Protein B1 (APBB1); Recombinant Amyloid Beta Precursor Protein Binding Protein B1 (APBB1); Fe65; APB-B1; RIR; Amyloid beta A4 precursor protein-binding family B member 1; APBB1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 710

• Applicable Applications for APBB1 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Gly267~Phe528
• Tag: N-terminal His-tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 5.1
• Subcellular Location: Cell Membrane. Cytoplasm. Nucleus. Cell Projection. Growth Cone. Nucleus Speckle.
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for APBB1 recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4502131
• NCBI GeneID: 322
• NCBI Accession #: NP_001155.1
• NCBI GenBank Nucleotide #: NM_001164.4
• UniProt Accession #: O00213; Q7Z324; Q96A93; A1E379; A6NH82; A6NL69; B7Z1J5; B7Z1J6; B7Z2Y0; D3DQT2; V9GYK0; V9GYT4
• Molecular Weight: 33.0kDa
• NCBI Official Full Name: amyloid beta A4 protein-binding family B member 1 isoform a
• NCBI Official Synonym Full Names: amyloid beta precursor protein binding family B member 1
• NCBI Official Symbol: APBB1
• NCBI Official Synonym Symbols: RIR; FE65; MGC:9072
• NCBI Protein Information: amyloid beta A4 precursor protein-binding family B member 1
• UniProt Protein Name: Amyloid beta A4 precursor protein-binding family B member 1
• Protein Family: Amyloid beta A4 precursor protein-binding family
• UniProt Gene Name: APBB1
• UniProt Synonym Gene Names: FE65; RIR

NCBI Description

The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

Fe65: adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on Y142 (H2AXpY142) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as TIP60, probably explains its trancription activation activity.

Protein type: Adaptor/scaffold; Apoptosis; Transcription regulation

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cytoplasm; growth cone; lamellipodium; nucleoplasm; nucleus; plasma membrane; synapse

Molecular Function: beta-amyloid binding; chromatin binding; histone binding; protein binding; transcription factor binding

Biological Process: cell cycle arrest; negative regulation of cell growth; negative regulation of thymidylate synthase biosynthetic process; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to DNA damage stimulus