Annexin V Recombinant Protein | ANXA5 recombinant protein

Annexin V, Recombinant, Human (Annexin A5, Endonexin II, Lipocortin V, Placental Anticoagulant Protein I, Calphobindin I, Vascular Anticoagulant-alpha)

Cat. Num. AAA637728

• Gene Names: ANXA5; PP4; ANX5; ENX2; RPRGL3
• Purity: Highly Purified; >95% (SDS-PAGE)
• Synonyms: Annexin V; Recombinant; Human (Annexin A5; Endonexin II; Lipocortin V; Placental Anticoagulant Protein I; Calphobindin I; Vascular Anticoagulant-alpha); ANXA5 recombinant protein

• Host: Human, E Coli
• Specificity: Binds to phosphatidylserine (PS).
• Purity/Purification: Highly Purified; >95% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS pH 7.2.
• Sequence: Recombinant human annexin V fused to a GST-tag.

• Preparation and Storage: Short-term Storage: +4 degree C; Long-term Storage: -20 degree C; Stable for at least 1 year after receipt when stored at -20 degree C. Reconstituted solutions can be kept at 4 degree C under sterile conditions for one week or should be frozen in aliquots at -20 degree C.

Related Product Information for ANXA5 recombinant protein

Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V competes for phosphatidylserine binding sites (e.g. platelets) with prothrombin or coagulation factors and inhibis the activity of phospholipase A1. Antibodies directed against annexin V are found in patients with a disease called the antiphospholipid syndrome(APS), a thrombophilic disease associated with autoantibodies against phospholipid compounds. Annexin V is used as a probe in the "annexin V affinity assay" to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis and other forms of cell death.

Product Categories/Family for ANXA5 recombinant protein

Molecular Biology; MB-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4502107
• NCBI GeneID: 308
• NCBI Accession #: NP_001145.1
• NCBI GenBank Nucleotide #: NM_001154.3
• UniProt Accession #: P08758; Q6FHB3; Q6FI16; Q8WV69; Q9UDH9; D3DNW7
• Molecular Weight: ~52kD (SDS-PAGE)
• NCBI Official Full Name: annexin A5
• NCBI Official Synonym Full Names: annexin A5
• NCBI Official Symbol: ANXA5
• NCBI Official Synonym Symbols: PP4; ANX5; ENX2; RPRGL3
• NCBI Protein Information: annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; thromboplastin inhibitor; vascular anticoagulant-alpha; placental anticoagulant protein 4; placental anticoagulant protein I
• UniProt Protein Name: Annexin A5
• Protein Family: Annexin
• UniProt Gene Name: ANXA5
• UniProt Synonym Gene Names: ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha
• UniProt Entry Name: ANXA5_HUMAN

NCBI Description

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA5: a calcium/phospholipid-binding protein and an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. The binding of labeled ANXA5 to phosphatidylserine is used as a marker of apoptosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Inhibitor; Apoptosis; Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: focal adhesion; membrane; cytoplasm; intracellular; external side of plasma membrane

Molecular Function: phospholipase inhibitor activity; calcium-dependent phospholipid binding; phospholipid binding; calcium ion binding

Biological Process: response to organic substance; negative regulation of catalytic activity; blood coagulation; negative regulation of coagulation; signal transduction; negative regulation of apoptosis

Disease: Pregnancy Loss, Recurrent, Susceptibility To, 3