Annexin V/ANXA5 Recombinant Protein | ANXA5 recombinant protein

Annexin V/ANXA5 Protein, Human, Recombinant

Cat. Num. AAA8120275

• Gene Names: ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Annexin V/ANXA5; Annexin V/ANXA5 Protein; Human; Recombinant; Human ANXA5/Annexin V/Annexin A5 Protein; ANX5 Protein; ENX2 Protein; HEL-S-7 Protein; PP4 Protein; RPRGL3 Protein; annexin A5; ANXA5 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 240mM NaCl, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp320

• Species: Human
• Predicted N Terminal: Met 1
• Tag: Native
• Protein Construction: A DNA sequence encoding the human ANXA5 (P08758)(Met 1-Asp 320) was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for ANXA5 recombinant protein

Background: The placental anticoagulant protein Annexin A5 (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Annexin A5 (ANXA5) is a protein abundantly expressed in normal placenta where it contributes to the healthy outcome of a pregnancy. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. ANXA5 could be used as a biomarker for the early detection of PE and for the prediction of its severity. ANXA5 as an embryonic anticoagulant that appears deficient in contiguous specter of thrombophilia-related pregnancy complications culminating more frequently in miscarriage in a maternal M2 carrier background. As a potential indicator for malignancy and lymphatic metastasis, ANXA5 overexpression increases in vitro migration and invasion of Hca-P cell, promotes in vivo malignancy, LNM rate and level of Hca-P-transplanted mice. Hereditary thrombophilias can impair vascular placental functions and predispose to the birth of small-for-gestational age (SGA) babies. The placental anticoagulant protein annexin A5 (ANXA5) may contribute to this process. A functional haplotype (M2) within the ANXA5 gene is associated with fetal loss and venous thrombosis.

References

Cederholm A, et al. (2007) Annexin A5 as a novel player in prevention of atherothrombosis in SLE and in the general population. Ann N Y Acad Sci. 1108: 96-103.Schlaepfer DD, et al. (1992) Inhibition of Protein Kinase C by AnnexinI¤. Biochemistry. 31: 1886-91.Vermes I, et al. (1995) A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin I¤. J Immunol Methods. 184 (1): 39-51.

NCBI and Uniprot Product Information

• NCBI GI #: 4502107
• NCBI GeneID: 308
• NCBI Accession #: NP_001145.1
• NCBI GenBank Nucleotide #: NM_001154.3
• UniProt Accession #: P08758; Q6FHB3; Q6FI16; Q8WV69; Q9UDH9; D3DNW7
• Molecular Weight: 35,937 Da
• NCBI Official Full Name: annexin A5
• NCBI Official Synonym Full Names: annexin A5
• NCBI Official Symbol: ANXA5
• NCBI Official Synonym Symbols: PP4; ANX5; ENX2; RPRGL3; HEL-S-7
• NCBI Protein Information: annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; thromboplastin inhibitor; vascular anticoagulant-alpha; epididymis secretory protein Li 7; placental anticoagulant protein 4; placental an
• UniProt Protein Name: Annexin A5
• Protein Family: Annexin
• UniProt Gene Name: ANXA5
• UniProt Synonym Gene Names: ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha
• UniProt Entry Name: ANXA5_HUMAN

NCBI Description

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA5: a calcium/phospholipid-binding protein and an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. The binding of labeled ANXA5 to phosphatidylserine is used as a marker of apoptosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Inhibitor; Lipid-binding; Apoptosis

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: focal adhesion; membrane; cytoplasm; intracellular; external side of plasma membrane

Molecular Function: phospholipase inhibitor activity; calcium-dependent phospholipid binding; phospholipid binding; calcium ion binding

Biological Process: response to organic substance; negative regulation of catalytic activity; negative regulation of coagulation; blood coagulation; signal transduction; negative regulation of apoptosis

Disease: Pregnancy Loss, Recurrent, Susceptibility To, 3