MUMPS VIRUS Antigen | N antigen

NATIVE MUMPS VIRUS

Cat. Num. AAA239128

• Applications: ELISA
• Synonyms: MUMPS VIRUS; NATIVE MUMPS VIRUS; N antigen

• Form/Format: Inactivated Pathogen; Inactivated Mumps virus - liquid
• Concentration: Total protein concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 553

• Applicable Applications for N antigen: ELISA (EIA)
• Preparation: Mumps virus, Enders strain, cultured in BSC-1 cells. Optimally infected cells are harvested, disrupted by sonication in culture medium and subjected to low speed centrifugation. The supernatant from the infected culture is concentrated by crossflow ultrafiltration. The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.
• Instructions For Use: This item should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.
• Buffer Solution: Culture medium 1969B
• Activity: Antigenic activity is 51.1% of internal reference batch
• Target Species: Viral
• Product Information: Native mumps virus preparation Native mumps virus contains a high concentration of virus and viral components. The preparation also contains some cellular material suspended in tissue culture medium (1969B).; ; Mumps virus is a member of the Paramyxoviridae family and is the causative agent of mumps. Mumps is most typically characterised by painful swelling of the salivary glands and fever. In some adult men, the virus might also infect the testicles, rarely causing sterility. The virus is spread by airborne droplets from the nose or throat and can have an incubation period of up to three weeks. Infected individuals are contagious from approximately 6 days before until about 9 days after the onset of symptoms. The disease is self-limiting and treatment involves controlling specific symptoms. An effective vaccine exists and may be given separately or as part of the MMR vaccine.
• Preparation and Storage: Store at -70°C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

Related Product Information for N antigen

Native mumps virus is a member of the Paramyxoviridae family and is the causative agent of mumps. Mumps is most typically characterised by painful swelling of the salivary glands and fever. In some adult men, the virus might also infect the testicles, rarely causing sterility. The virus is spread by airborne droplets from the nose or throat and can have an incubation period of up to three weeks. Infected individuals are contagious from approximately 6 days before until about 9 days after the onset of symptoms. The disease is self-limiting and treatment involves controlling specific symptoms. An effective vaccine exists and may be given separately or as part of the MMR vaccine. Native mumps virus contains a high concentration of virus and viral components. The preparation also contains some cellular material suspended in 1969B.

NCBI and Uniprot Product Information

• NCBI GI #: 127906
• UniProt Accession #: P21277
• NCBI Official Full Name: Nucleoprotein
• UniProt Protein Name: Nucleoprotein
• UniProt Gene Name: N
• UniProt Synonym Gene Names: NP; NP; Protein N
• UniProt Entry Name: NCAP_MUMP1

Uniprot Description

Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases ().