APC Antibody | anti-APC antibody

APC (c-APC 28.9) Antibody

Cat. Num. AAA440098

• Gene Names: APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Synonyms: APC; APC (c-APC 28.9) Antibody; anti-APC antibody

• Specificity: Detects APC of human and mouse origin
• Form/Format: 100 ug/ml of mouse monoclonal IgG1 in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.100 mg/ml (varies by lot)
• Sequence Length: 2843

• Applicable Applications for anti-APC antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin, Immunofluorescence (IF)
• Application Notes: Western blotting starting dilution: 1:200. Positive control: colon cell line HCT116 lysates.
• Immunogen: Human APC carboxy terminal fusion with MBP
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Related Product Information for anti-APC antibody

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. APC is characterized by numerous polyps found throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations (1-3).

References

1.Luk GD. 1995. Diagnosis and therapy of hereditary polyposis syndromes. Gastroenterologist 3:153-167.2.Olschwag S, Laurent-Puig P, Melot T, Thuille B, and Thomas G. 1995. High resolution genetic map of the adenomatous polyposis coli gene (APC). Amer. J. Med. Gen. 56: 413-419.3.Harach HR, Williams GT, and Williams ED. 1994. Familial adenomatous polyposis associated thyroid carcinoma: a distinct type of follicular cell neoplasm. Histopathology 25: 549-561.

NCBI and Uniprot Product Information

• NCBI GI #: 53759122
• NCBI GeneID: 324
• NCBI Accession #: NP_000029.2
• NCBI GenBank Nucleotide #: NM_000038.5
• UniProt Accession #: P25054; Q15162; Q15163; Q93042; D3DT03
• Molecular Weight: 300,439 Da
• NCBI Official Full Name: adenomatous polyposis coli protein isoform b
• NCBI Official Synonym Full Names: adenomatous polyposis coli
• NCBI Official Symbol: APC
• NCBI Official Synonym Symbols: GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• NCBI Protein Information: adenomatous polyposis coli protein; adenomatosis polyposis coli tumor suppressor; deleted in polyposis 2.5; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: APC-related protein
• UniProt Gene Name: APC
• UniProt Synonym Gene Names: DP2.5; Protein APC
• UniProt Entry Name: APC_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; cytosol; beta-catenin destruction complex; nucleoplasm; kinetochore; cell-cell adherens junction; cytoplasmic microtubule; lamellipodium; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; positive regulation of apoptosis; apoptosis; somatic stem cell maintenance; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; muscle maintenance; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of osteoclast differentiation; cytokinesis after mitosis; hair follicle development; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell adhesion; cell cycle arrest; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; negative regulation of odontogenesis; regulation of attachment of spindle microtubules to kinetochore; cell migration; regulation of osteoblast differentiation; thymus development; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; dorsal/ventral pattern formation; axonogenesis; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma