Phleomycin, Streptomyces verticillus Antibiotic
Phleomycin, Streptomyces verticillus
Purity
Highly Purified~90%
Synonyms
Phleomycin; Streptomyces verticillus; Streptomyces verticillus antibiotic
Purity/Purification
Highly Purified
~90%
~90%
Form/Format
Supplied as a lyophilized powder.
Application Notes
Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13.665kD protein. The protein binds to the antibiotic and prevents binding to DNA., , Suggested Concentration for use:, Organism:, E. coli: 25-50ug/ml in Low Salt LB medium, Yeast: 50-300ug/ml in YPD or minimal medium, Mammalian Cells: 25-1000ug/ml (varies with cell line)
CAS Number
11006-33-0
Molecular Formula
C55H83N19O21S2Cu
Solubility
Soluble in water.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for up to 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Long term stability in solution has not been evaluated for this product. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Phleomycin, Streptomyces verticillus antibiotic
This unique antibiotic shows high toxicity in mammalian cells, plants, yeasts and bacteria. Ocin acts by binding to DNA and cleaving it causing cell death. Ocin is a copper-chelated glycopeptide antibiotic isolated from culture broth of a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics.
The product of Sh ble, (Streptoalloteichus hindustanus bleomycin-resistance) gene confers resistance to Zeocin and Zeocell. The 14kD protein stoichiometrically binds to Zeocin and inhibits DNA strand cleavage and subsequent cell death.
Produced by a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13,665 Dalton protein. The protein binds to the antibiotic and prevents binding to DNA.
Phleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticullus. It binds and intercalates DNA thus destroying the integrity of the double helix. Use of phleomycin is recommended for cells poorly sensitive to Zeocin. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.
The product of Sh ble, (Streptoalloteichus hindustanus bleomycin-resistance) gene confers resistance to Zeocin and Zeocell. The 14kD protein stoichiometrically binds to Zeocin and inhibits DNA strand cleavage and subsequent cell death.
Produced by a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13,665 Dalton protein. The protein binds to the antibiotic and prevents binding to DNA.
Phleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticullus. It binds and intercalates DNA thus destroying the integrity of the double helix. Use of phleomycin is recommended for cells poorly sensitive to Zeocin. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.
Product Categories/Family for Phleomycin, Streptomyces verticillus antibiotic
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Product Notes
The Phleomycin, Streptomyces verticillus (Catalog #AAA651416) is an Antibiotic and is intended for research purposes only. The product is available for immediate purchase. Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13.665kD protein. The protein binds to the antibiotic and prevents binding to DNA.,, Suggested Concentration for use:, Organism:, E. coli: 25-50ug/ml in Low Salt LB medium, Yeast: 50-300ug/ml in YPD or minimal medium, Mammalian Cells: 25-1000ug/ml (varies with cell line). Researchers should empirically determine the suitability of the Phleomycin, Streptomyces verticillus for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phleomycin, Streptomyces verticillus, Antibiotic" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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