Rat Acidic leucine-rich nuclear phosphoprotein 32 family member B ELISA Kit | ANP32B elisa kit

Rat Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) ELISA Kit

Cat. Num. AAA283519

• Gene Names: Anp32b; PAL31
• Reactivity: Rat
• Synonyms: Acidic leucine-rich nuclear phosphoprotein 32 family member B; Rat Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) ELISA Kit; ANP32B elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat ANP32B. No significant cross-reactivity or interference between Rat ANP32B and analogues was observed.
• Sequence Length: 251

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ANP32B elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ANP32B in samples. An antibody specific for ANP32B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANP32B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANP32B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANP32B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 18777770
• NCBI GeneID: 170724
• NCBI Accession #: NP_571986.1
• NCBI GenBank Nucleotide #: NM_131911.2
• UniProt Accession #: Q9EST6
• Molecular Weight: 31,061 Da
• NCBI Official Full Name: acidic leucine-rich nuclear phosphoprotein 32 family member B
• NCBI Official Synonym Full Names: acidic nuclear phosphoprotein 32 family member B
• NCBI Official Symbol: Anp32b
• NCBI Official Synonym Symbols: PAL31
• NCBI Protein Information: acidic leucine-rich nuclear phosphoprotein 32 family member B
• UniProt Protein Name: Acidic leucine-rich nuclear phosphoprotein 32 family member B
• Protein Family: Acidic leucine-rich nuclear phosphoprotein 32 family
• UniProt Gene Name: Anp32b
• UniProt Synonym Gene Names: Pal31

NCBI Description

may play a role in cell cycle control and in signaling of lymphocyte proliferation [RGD, Feb 2006]

Uniprot Description

ANP32B: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity. Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Belongs to the ANP32 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5q22

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: histone binding; protein binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; histone exchange; inner ear development; negative regulation of apoptosis; negative regulation of cell differentiation; nucleocytoplasmic transport; nucleosome assembly; palate development; positive regulation of cell proliferation; positive regulation of protein export from nucleus; regulation of caspase activity; vasculature development; ventricular system development