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SDS-Page

ANKRD1 recombinant protein

ANKRD1, 1-319aa, Human, His tag, E Coli

Gene Names
ANKRD1; ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
ANKRD1; 1-319aa; Human; His tag; E Coli; Ankyrin repeat domain-containing protein 1; ALRP; bA320F15.2; C-193; CARP; CVARP; MCARP; ANKRD1 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 5mM DTT
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMMVLKVE ELVTGKKNGN GEAGEFLPED FRDGEYEAAV TLEKQEDLKT LLAHPVTLGE QQWKSEKQRE AELKKKKLEQ RSKLENLEDL EIIIQLKKRK KYRKTKVPVV KEPEPEIITE PVDVPTFLKA ALENKLPVVE KFLSDKNNPD VCDEYKRTAL HRACLEGHLA IVEKLMEAGA QIEFRDMLES TAIHWASRGG NLDVLKLLLN KGAKISARDK LLSTALHVAV RTGHYECAEH LIACEADLNA KDREGDTPLH DAVRLNRYKM IRLLIMYGAD LNIKNCAGKT PMDLVLHWQN GTKAIFDSLR ENSYKTSRIA TF
Sequence Length
319
Applicable Applications for ANKRD1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for ANKRD1 recombinant protein
Ankyrin repeat domain-containing protein 1, also known as ANKRD1, may play an important role in endothelial cell activation. This protein may act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Recombinant human ANKRD1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for ANKRD1 recombinant protein
References
Chu W., et al. (1995) J. Biol. Chem. J. Biol. Chem. 270:10236-10245; Park J.-H., et al. (2005) Cancer Res. 65:2804-2814

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38.6 kDa (342aa) confirmed by MALDI-TOF
NCBI Official Full Name
ankyrin repeat domain-containing protein 1
NCBI Official Synonym Full Names
ankyrin repeat domain 1
NCBI Official Symbol
ANKRD1
NCBI Official Synonym Symbols
ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
NCBI Protein Information
ankyrin repeat domain-containing protein 1
UniProt Protein Name
Ankyrin repeat domain-containing protein 1
UniProt Gene Name
ANKRD1
UniProt Synonym Gene Names
C193; CARP; HA1A2

NCBI Description

The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system. [provided by RefSeq, Jul 2008]

Uniprot Description

May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.

Research Articles on ANKRD1

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Product Notes

The ANKRD1 ankrd1 (Catalog #AAA204181) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ANKRD1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ANKRD1 ankrd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMMVLKVE ELVTGKKNGN GEAGEFLPED FRDGEYEAAV TLEKQEDLKT LLAHPVTLGE QQWKSEKQRE AELKKKKLEQ RSKLENLEDL EIIIQLKKRK KYRKTKVPVV KEPEPEIITE PVDVPTFLKA ALENKLPVVE KFLSDKNNPD VCDEYKRTAL HRACLEGHLA IVEKLMEAGA QIEFRDMLES TAIHWASRGG NLDVLKLLLN KGAKISARDK LLSTALHVAV RTGHYECAEH LIACEADLNA KDREGDTPLH DAVRLNRYKM IRLLIMYGAD LNIKNCAGKT PMDLVLHWQN GTKAIFDSLR ENSYKTSRIA TF. It is sometimes possible for the material contained within the vial of "ANKRD1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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