Angiopoietin Like Protein 4 Recombinant Protein | ANGPTL4 recombinant protein

Recombinant Angiopoietin Like Protein 4 (ANGPTL4)

Cat. Num. AAA2032932

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Angiopoietin Like Protein 4; Recombinant Angiopoietin Like Protein 4 (ANGPTL4); ARP4; FIAF; HFARP; NL2; PGAR; Pp1158; Fasting-Induced Adipose Factor; Hepatic Angiopoietin-Related Protein; Hepatic Fibrinogen/Angiopoietin-Related Protein; ANGPTL4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: RRL NGSVDFNQSW EAYKDGFGDP QGEFWLGLEK MHSITGDRGS QLAVQLQDWD GNAKLLQFPI HLGGEDTAYS LQLTEPTANE LGATNVSPNG LSLPFSTWDQ DHDLRGDLNCAKSLSGGWWF GTCSHSNLNG QYFHSIPRQR QQRKKGIFWK TWKGRYYPL
• Sequence Length: 405

• Applicable Applications for ANGPTL4 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 22.1kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Arg228~Leu389
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 40018598
• NCBI GeneID: 362850
• NCBI Accession #: NP_954546.1
• NCBI GenBank Nucleotide #: NM_199115.2
• Molecular Weight: 44,951 Da
• NCBI Official Full Name: angiopoietin-related protein 4
• NCBI Official Synonym Full Names: angiopoietin-like 4
• NCBI Official Symbol: Angptl4
• NCBI Protein Information: angiopoietin-related protein 4
• UniProt Protein Name: Angiopoietin-related protein 4
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: Angptl4
• UniProt Synonym Gene Names: HFARP
• UniProt Entry Name: ANGL4_RAT

NCBI Description

a circulating protein which causes an increase in plasma very low density lipoprotein by inhibition of lipoprotein lipase activity [RGD, Feb 2006]

Uniprot Description

Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation ().