Chicken Angiopoietin Like Protein 4 ELISA Kit | ANGPTL4 elisa kit

Chicken Angiopoietin Like Protein 4 ELISA Kit

Cat. Num. AAA736198

• Gene Names: ANGPTL4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
• Reactivity: Chicken
• Synonyms: Angiopoietin Like Protein 4; Chicken Angiopoietin Like Protein 4 ELISA Kit; ANGPTL4 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This ANGPTL4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ANGPTL4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPTL4 elisa kit

Principle of the Assay: ANGPTL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANGPTL4 antibody and an ANGPTL4-HRP conjugate. The assay sample and buffer are incubated together with ANGPTL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANGPTL4 concentration since ANGPTL4 from samples and ANGPTL4-HRP conjugate compete for the anti-ANGPTL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANGPTL4 from the sample, fewer sites are left to bind ANGPTL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANGPTL4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ANGPTL4 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 89264696
• NCBI GeneID: 51129
• NCBI Accession #: NP_001034756.1
• NCBI GenBank Nucleotide #: NM_001039667.2
• Molecular Weight: 26,853 Da
• NCBI Official Full Name: angiopoietin-related protein 4 isoform b
• NCBI Official Synonym Full Names: angiopoietin-like 4
• NCBI Official Symbol: ANGPTL4
• NCBI Official Synonym Symbols: NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
• NCBI Protein Information: angiopoietin-related protein 4; fasting-induced adipose factor; PPARG angiopoietin related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein
• UniProt Protein Name: Angiopoietin-related protein 4
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL4
• UniProt Synonym Gene Names: ARP4; HFARP; PGAR; PP1158; PSEC0166; UNQ171/PRO197; HFARP
• UniProt Entry Name: ANGL4_HUMAN

NCBI Description

This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013]

Uniprot Description

ANGPTL4: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

Protein type: Secreted; Apoptosis; Extracellular matrix; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: enzyme inhibitor activity; protein binding

Biological Process: positive regulation of angiogenesis; response to hypoxia; negative regulation of lipoprotein lipase activity; angiogenesis; cellular lipid metabolic process; cell differentiation; protein homooligomerization; negative regulation of apoptosis

Disease: Plasma Triglyceride Level Quantitative Trait Locus