Standard Curve (Sample)
(Rat CCL7/MCP-3 PicoKine ELISA Kit standard curve)
Rat CCL7/MCP-3 ELISA Kit | CCL7 elisa kit
Rat CCL7/MCP-3 PicoKine ELISA Kit
Reactivity
Rat
Synonyms
CCL7/MCP-3; N/A; Rat CCL7/MCP-3 PicoKine ELISA Kit; C-C motif chemokine 7; chemokine (C-C motif) ligand 7; Ccl7; CCL7_HUMAN; Chemokine CC Motif Ligand 7; Chemotactic protein 3; FIC; Intercrine/chemokine MARC; MARC; MCP 3; MCP-3; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RANTES; sis homolog; RP23-350G1.4; SCYA6; SCYA7; Small inducible cytokine A7; Small-inducible cytokine A7; CCL7 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat CCL7
Sequence Length
97
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Rat CCL7/MCP-3 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Rat CCL7/MCP-3 PicoKine ELISA Kit standard curve)
Related Product Information for CCL7 elisa kit
Principle of the assay: rat CCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL7 has been precoated onto 96-well plates. Standards(E.coli, Q24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CCL7 amount of sample captured in plate.
Background: Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
Background: Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
10,906 Da
NCBI Official Full Name
C-C motif chemokine 7
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 7
NCBI Official Symbol
Ccl7
NCBI Protein Information
C-C motif chemokine 7; MCP-3; chemotactic protein-3; small-inducible cytokine A7; monocyte chemotactic protein 3; monocyte chemoattractant protein 3
UniProt Protein Name
C-C motif chemokine 7
UniProt Gene Name
Ccl7
UniProt Synonym Gene Names
Mcp3; Scya7; MCP-3
UniProt Entry Name
CCL7_RAT
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Product Notes
The Rat CCL7 ccl7 (Catalog #AAA45838) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45838 ELISA Kit recognizes Rat CCL7. It is sometimes possible for the material contained within the vial of "CCL7/MCP-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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