Typical Testing Data/Standard Curve (for reference only)
Rat Anti-nuclear ELISA Kit | ANA elisa kit
Rat Anti-nuclear Antibody ELISA Kit
Gene Names
BTG3; ANA; TOB5; TOFA; TOB55; MGC8928
Reactivity
Rat
Synonyms
Anti-nuclear; Rat Anti-nuclear Antibody ELISA Kit; Anti-nuclear Antibody (Rat); ANA elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This ANAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ANAb. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ANA elisa kit
Principle of the assay: ANAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Nuclear antigen and an ANAb-HRP conjugate. The assay sample and buffer are incubated together with ANAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANAb concentration since ANAb from samples and ANAb-HRP conjugate compete for the Nuclear antigen binding site. Since the number of sites is limited, as more sites are occupied by ANAb from the sample, fewer sites are left to bind ANAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANAb concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ANA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,116 Da
NCBI Official Full Name
ANA
NCBI Official Synonym Full Names
BTG family, member 3
NCBI Official Symbol
BTG3
NCBI Official Synonym Symbols
ANA; TOB5; TOFA; TOB55; MGC8928
NCBI Protein Information
protein BTG3; protein Tob5; OTTHUMP00000083229; OTTHUMP00000083230; B-cell translocation gene 3; abundant in neuroepithelium area protein
UniProt Protein Name
Protein BTG3
Protein Family
UniProt Gene Name
BTG3
UniProt Synonym Gene Names
ANA; TOB5
UniProt Entry Name
BTG3_HUMAN
NCBI Description
The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
BTG3: Overexpression impairs serum-induced cell cycle progression from the G0/G1 to S phase. Belongs to the BTG family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 21q21.1
Cellular Component: cytoplasm
Molecular Function: protein binding
Biological Process: negative regulation of cell proliferation; negative regulation of mitotic cell cycle
Research Articles on ANA
Similar Products
Product Notes
The Rat ANA btg3 (Catalog #AAA730007) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730007 ELISA Kit recognizes Rat ANA. It is sometimes possible for the material contained within the vial of "Anti-nuclear, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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