Fish Amylase Alpha ELISA Kit | Amyalpha elisa kit

Fish Amylase Alpha ELISA Kit

Cat. Num. AAA77098

• Gene Names: AMY2A; PA; AMY2; AMY2B
• Reactivity: Fish
• Synonyms: Amylase Alpha; Fish Amylase Alpha ELISA Kit; Amyalpha elisa kit

• Reactivity: Fish
• Specificity: No significant cross-reactivity or interference between Fish Amyalpha and analogues was observed.
• Sequence Length: 511

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 15.6U/L-500U/L.
• Sensitivity: 2.0U/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated Amyalpha concentrations in Fish serum, plasma and other body fluids. Using Purified Fish Amyalpha antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Amyalpha and Amyalpha antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Amyalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for Amyalpha elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated Amyalpha concentrations in Fish serum, plasma and other body fluids. Using Purified Fish Amyalpha antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Amyalpha and Amyalpha antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Amyalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 529397
• NCBI GeneID: 279
• UniProt Accession #: P04746; Q9UBH3
• Molecular Weight: 57,707 Da
• NCBI Official Full Name: amylase
• NCBI Official Synonym Full Names: amylase, alpha 2A (pancreatic)
• NCBI Official Symbol: AMY2A
• NCBI Official Synonym Symbols: PA; AMY2; AMY2B
• NCBI Protein Information: pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase
• UniProt Protein Name: Pancreatic alpha-amylase
• UniProt Gene Name: AMY2A
• UniProt Synonym Gene Names: PA
• UniProt Entry Name: AMYP_HUMAN

NCBI Description

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide; EC 3.2.1.1; Hydrolase; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space; extracellular region

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis