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SDS-Page (Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

AMPKG3 recombinant protein

AMPKG3 Protein

Applications
Western Blot
Synonyms
AMPKG3; AMPKG3 Protein; PRKAG3; AMPKG3 recombinant protein
Ordering
For Research Use Only!
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for AMPKG3 recombinant protein
Western Blot (WB)
Tag
GST/HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

SDS-Page (Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for AMPKG3 recombinant protein
Recombinant full-length human AMPKG3 subunit was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags. The gene accession numbers is NM_017431.
5'-AMP-activated protein kinase subunit gamma-3 (AMPKG3) is an AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. Ablation of the gamma3 subunit is associated with impaired glycogen resynthesis in skeletal muscle In contrast, naturally occurring mutations in the gamma3 subunit increase glycogen storage in skeletal muscle, which may enhance glycogen storage by shifting the metabolic fate of glucose from oxidation to storage.
References
1. Barnes BR, et al. 5'-AMP-activated protein kinase regulates skeletal muscle glycogen content and ergogenics. FASEB J. 19:773-9, 2005.
2. Mu J, et al. A role for AMP-activated protein kinase in contraction-and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell. 7:1085-94, 2001.

NCBI and Uniprot Product Information

NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~96 -108kDa

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Product Notes

The AMPKG3 (Catalog #AAA516572) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AMPKG3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the AMPKG3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPKG3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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